FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17353494
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230230.1326706886809077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26300.09.21439551
TATACTG21050.08.9765015
CCTATAC13500.08.812583
TAGACTG23350.08.6317935
TACACTG29650.08.5915625
GTGTATA29000.08.4048071
CTACACT28900.08.136114
CTAGGAC26400.08.0055463
GTGTAGA24850.07.8918371
GTCTAAG17050.07.805051
CTAATAC19800.07.70511673
TAATACC16700.07.6265894
GTGTAAG23700.07.56550841
TAGACAG31100.07.56091455
GTGTAGG29950.07.5301831
CCAGGAC59700.07.52572973
GTCTAGG24850.07.49724531
CCTACAC27650.07.49177073
ATACTGT28250.07.4816996
GTACTAG10350.07.4438711
Produced by FastQC (version 0.11.5)