FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16032037
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG5050.010.2571115
TATACTG19100.09.4552065
TAGACAG33350.08.7733435
ACGAGAC10800.08.42619222
TAGACTG21650.08.2122195
TATACCG2756.992959E-78.1452045
TACACTG25950.07.93039235
TAGCCCG10600.07.79221345
GTCCTAG22250.07.6765281
CTAGTAC9150.07.64999063
GTCTAGA20700.07.6426351
TATTCCG4604.7293724E-117.6084625
TATACAC29850.07.55080943
CTAGGAC24750.07.466393
GTATAGG13550.07.4392341
GGACCGT9800.07.4283896
TTAGACT17850.07.37232264
AACCGTC6300.07.33313047
CTTAGAC15300.07.3199913
CCTATAC12050.07.31923153
Produced by FastQC (version 0.11.5)