FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16387455
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA36500.010.4513321
TGTATAA33050.09.3706232
GTATAAG30200.09.1820123
TAGACCG4600.09.1291045
TATACCG2151.8309243E-58.4638825
TAGGACG6400.08.3134464
TAGGACC15400.08.1827494
TATAAGA35750.08.1072164
GTCCTAG21500.07.8277761
TACACTG26550.07.64483645
GTCTAGA19800.07.57903961
CCTATAC12050.07.55450253
CTAGGAC23250.07.52950863
GTGTAGG23800.07.4838031
TAGGTCG3752.4307155E-87.4655785
TACACAG36800.07.3793595
TATACTG18250.07.363315
AGGACCG16350.07.3628415
GTCCTAA16350.07.2911621
GTATAGG11550.07.28559161
Produced by FastQC (version 0.11.5)