FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16234231
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201120.12388637318269033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG20050.09.4956765
TCTATAC13000.09.3652443
TTAGACT16150.08.753264
TATACTG17600.08.351765
TAGACAG25850.08.3399135
TACACCG8500.08.2347895
CTAGACT19900.08.1587924
CGTATAC4503.6379788E-128.0854483
TCTAGAC16050.08.0215073
CTTATAC14150.07.9118273
TACACTG27050.07.86643035
GTATAGA15000.07.83538341
CCTACAC27300.07.7402953
CTAGGAC25750.07.71710443
GTATACG4751.2732926E-117.65864471
TAGGACT23800.07.64517354
TATGCCG3505.705988E-87.5995335
AGGACCG17250.07.4662085
GTGTAGG25150.07.4548521
CTATACT19650.07.4078424
Produced by FastQC (version 0.11.5)