FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16518436
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190860.11554362652735405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG6850.08.9915555
GTCTAGA16850.08.88941
CTAGTAC9700.08.2232893
GTATTAG16150.08.1478661
CCTATAC14300.08.1224013
GTCTACG5900.08.0670631
AGGACCG17500.07.9989825
TATACTG18550.07.9989825
GTACTAG8850.07.9088861
TCTATAC12750.07.9025033
TAGACCG6750.07.8804795
GGACCGT10500.07.8669546
TACACTG28800.07.77678785
CTACACT28150.07.75655564
GTCCTAG26200.07.74738741
GTGTTAG25950.07.660191
GTGTAGG25250.07.65078971
CTATACT18550.07.6207934
CTTACAC23750.07.54203843
TAGGACC16600.07.5042044
Produced by FastQC (version 0.11.5)