FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800267
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCGAG270486496.59307487464588No Hit
AGCTCGCG285471.0194385035426978No Hit
AGCTCCAG59470.21237260589793758No Hit
AGCACGAG57350.2048018992474646No Hit
CGCTCGAG54910.19608844442333537No Hit
AGCTCGAC48420.17291208302636854No Hit
AGCTCGAA45450.16230595153962105No Hit
AGCGCGAG43860.15662792155176633No Hit
AGCTCGTG40130.1433077631525851No Hit
AGCTCGAT35620.12720215608011665No Hit
AGCTAGAG32180.11491761321331144No Hit
ACCTCGAG31790.11352488887666783No Hit
AGCTGGAG31090.11102512724679468No Hit
TGCTCGAG30770.10988237907313839No Hit
AGATCGAG30300.10820396769308069No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)