FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7637921
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA740063296.89327763405775No Hit
GTGGCGCA735070.9623953952914673No Hit
GTGGAGCC202060.264548428819832No Hit
GGGGAGCA162430.21266258187273737No Hit
GTGGAGCT125010.16367019245158465No Hit
GAGGAGCA119800.15684896452843647No Hit
GTGGACCA99170.12983899676364813ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGGAGAA92180.12068729173815754No Hit
GTGGAGGA89270.11687735445286747No Hit
GTGGTGCA87540.11461234019047853No Hit
GTGCAGCA86880.11374823070309314No Hit
CTGGAGCA84290.1103572555935051No Hit
GTGTAGCA82080.10746379806756315No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)