FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15159250
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG17100.09.414935
TACACTG26200.09.3508725
TAGACTG19500.08.4715485
TACACCG6450.08.2478215
TAGGACT20950.08.21748354
CTAGGAC23150.08.0997943
TAGACAG22900.08.00855
CTACACT25900.07.9979694
TAGGACC14300.07.92805624
TAATACT18650.07.88003644
GTACTAG9350.07.7813681
CCTACAC22950.07.74356943
GTATAGG12700.07.71184871
GTGTAAG21450.07.69695431
CTAGACT20050.07.60904034
GTCCTAG20450.07.5944071
TTAGGAC13100.07.4773363
TAATACG3609.399628E-87.3870134
GTACTGT16750.07.35556846
TACACAG30300.07.34632065
Produced by FastQC (version 0.11.5)