FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147465
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT340401.0815052748799432No Hit
TGCTGCTACCGTTCTC309200.9823778818827215No Hit
AAACGACCTCTTCAAG305520.9706859329651004No Hit
ATGGGTTACAATCGAA297540.9453321959100419No Hit
TATGCTTTGGACTGTT288960.918072162835806No Hit
GCCTCTTGATCTGTGA282800.8985008570389187No Hit
AAAGCGGACTCGGGCT269500.856244628613821No Hit
TCCAAAGTGCTCACGG257600.8184364242334704No Hit
GCAGGTCTGGCTGTTA253540.8055371545037039No Hit
TAGTGCATGAACCCGC242430.7702389065486034No Hit
GAAGTATGAACAGGTG238220.7568630628140424No Hit
TAACTCACTGGTGTCC234750.7458383175031335No Hit
TGTGGCTCTTACGATC230820.7333520785775218No Hit
TTCCCATCTCTTTGTT204540.6498563129375545No Hit
GAGCCTGACCGAGTTT202400.6430571904691553No Hit
TCACCTGACTCAATGC196830.6253604090911257No Hit
GGAACTACTACCAAGG195500.621134786248616No Hit
TGAGAATGAGCAGTTT186140.5913965683494494No Hit
CTAGAGACTAATCTGC185880.5905705067411393No Hit
ATGTCCAGAGTCGGTC185500.5893631859289937No Hit
GAAGTAATGAGTCTGT185340.5888548403238797No Hit
TCGTATACTGCTAATC177450.5637870476716977No Hit
TGCGGTGACCTTAGTG175190.5566066659994631No Hit
TCACACAGATTGGGAG174680.5549863143831624No Hit
TTCGTCCGACCTGTGG172460.5479330191122062No Hit
ATTCCTAACTGCTACA169810.5395135450275063No Hit
TCCGCGAGAGAACAGC168270.5346207185782844No Hit
ACTGATTCTCGGCTCA167790.5330956817629425No Hit
AGGTCGAGAAGGGAGG166800.5299502933313No Hit
TGGACCTCTAGGGAGG163110.5182265728133594No Hit
TGGCCTGGACCCGAGT162030.5147952399788401No Hit
CCATGATGATTCGGGC153830.4887425277167498No Hit
GCAATCATGGAACGTG152340.48400855926912606No Hit
TTCGATGGAAATCCTG142600.45306302055781394No Hit
ACGAGGATGGAGCTGA140160.4453107500798262No Hit
AAGGTGCGAACAGATA136090.4323797087497399No Hit
TCCCAGACTGTCTTGA132080.4196392970215713No Hit
TGGTCCTTGGAGAGGG131000.4162079641870521No Hit
TTTCGTAACAGTACGG130640.41506418657554567No Hit
GCGGTTCGAGTACGTT127740.40585042248285524No Hit
TCTCTCTACTCTGGAT125480.39867004081062063No Hit
TCCTTCACTTGTCACA113180.3595909724174852No Hit
TCGCCGAGAAGGTAAT111580.3545075163663456No Hit
AGCCCAGTGAAAGTAC110220.350186578722877No Hit
TTCGATGGACAGGTGA110180.3500594923215985No Hit
TGATATCCTCGATGTT108340.34421351786278803No Hit
AGCTGCTCTTGTCACA106740.3391300618116484No Hit
AACTCCCTGCTTCACC106560.3385581730058952No Hit
CTTTCGGTGCTTTGTT105500.3351903833720153No Hit
AACCCTCACGCGGTTC104330.33147310613461944No Hit
ATGGCCGTGATACGCT101980.3240067800595082No Hit
CCCTTGCCTCCTACGG100650.31978115721699846No Hit
TCGGCTTACCTCCTAC97520.3098366463169566No Hit
TGTTTGTTGCAAGTTC92370.29347427215235117No Hit
ACCCTTGTGGGCCATC91970.2922034081395663No Hit
AAACACGCTGCAGTTT91340.2902017973194301No Hit
TGGCCTTTGCGAAAGC90420.28727881009002487No Hit
GGGGGGGGGGGGGGGG89930.28572200167436335No Hit
CTAACTGGAACCTTGA88860.28232244044016375No Hit
AAATAGGACTCTATCT86790.2757457191740019No Hit
TACAACGTGAGCAGGT86510.27485611436505253No Hit
CAATGTGTGGTTGACT85800.27260033074235934No Hit
TCAAAGGCTCTTAGTG85060.2702492323187073No Hit
CTTTCGGTGAGCCATT80720.2564603577799912No Hit
TGAACTGTGTGCTGGC78150.24829505649784828No Hit
AGTGTTCGATTCGCCT74670.2372385395866197No Hit
GAGGTTGGAGGAATAT74630.23711145318534121No Hit
CAGCCGATGTAATAGG73710.23418846595593595No Hit
GGCTTGCGAATACGCT72300.22970867031086922No Hit
ACAGCCGACAAACATG71140.22602316467379302No Hit
GATAAGAACATTGGTC69830.2218610850319225No Hit
GTGCTAATGGGAGTCA66740.21204366053315923No Hit
CGACGTTCTTGCTACA66630.21169417292964338No Hit
GTAAAGCCTGAAATCA65330.2075638648880925No Hit
TTGGGAGGAATCTGTG63510.2017814336299212No Hit
TGTACTGGAAATGCTC63470.20165434722864273No Hit
GGACATTTGGCCGCTA62600.19889021800083556No Hit
GGGTGAAACCGACTAT59640.1894858243062274No Hit
AGGGACCCTAATCCTG59280.188342046694721No Hit
GGAACATCTTTACGAC59220.18815141709280325No Hit
TAACGAGCTTACTTTC58850.18697586788097723No Hit
GGACCACTGGCTAGAG57920.18402110905125235No Hit
TCTTGAACTCGTACAT57460.18255961543654972No Hit
CGGATGAGAACAGGCA56770.1803673750144958No Hit
TACGTGTGAAACGCAG55340.1758240361687898No Hit
TACCACCTGAAACATG54370.17274219093778645No Hit
AGCTTGTGAACCGTGC53750.17077235171796987No Hit
CATGATGTGCCTAGTA52690.1674045620840899No Hit
ACTTATCTGTCAGGTT52410.16651495727514048No Hit
AACTAGCACTGCCACG52310.16619724127194424No Hit
ACGTGTTGATCTTTGT49530.15736473638308926No Hit
TCGGACCGAATGTCCA48300.1534568295437757No Hit
AACTACGACATTGCCA48280.15339328634313645No Hit
GTCCGAGTGGTCTTGA47830.15196356432875346No Hit
TGGTAGGGATGTGCTA47460.1507880151169274No Hit
CTCGGATCTAGTACGG46440.14754731188432596No Hit
GTAGATAGAGCTGAGA46400.14742022548304745No Hit
GGTAGTTTGTCCAGCG45930.14592696026802524No Hit
AACGAACCTGACACCC45890.14579987386674673No Hit
TTTAGGGACTGGGCAA45480.14449723825364222No Hit
CGTCGTACTTGCGCCA45400.14424306545108523No Hit
CCTTCAGTGCAACGCC44220.1404940166133698No Hit
AGTTTGCGAGCAGGAT43520.13827000459099625No Hit
GCCATAATGACCGTTA43210.13728508498108796No Hit
TACCACCTGTACTTTC43150.13709445537917023No Hit
TGAGGAGTGGTCCCAG42400.13471158535519856No Hit
AGAACGCGACACAGTT41820.13286883253666046No Hit
CAGGCCATGTCGTAGT41250.13105785131844197No Hit
CTTTAACTGTTCGCCT41160.13077190691556537No Hit
GAGAAGAGATGCTACA40340.12816663568935635No Hit
GTCCGTTACGGATGGT39530.12559313606346695No Hit
GGGATGTGATGCATGT39240.12467175965419791No Hit
CTTACATTGGAAGGCT39020.1239727844471662No Hit
GAACCATACTTTGGGT38890.12355975364301112No Hit
TGCAATGACTCCGTAA38070.12095448241680208No Hit
GAAGGACACCCAGCTG37840.12022373560945077No Hit
GAAAGTCCTTATCTGG37390.11879401359506778No Hit
CCGTTACTGTTGGTTG36910.11726897677972592No Hit
CGATCGGTGCGAGGAT36900.11723720517940628No Hit
TCCTCATCTTTCTGGA36360.11552153876214669No Hit
GGCTTAAACTGCATCA36160.11488610675575424No Hit
AACTACGACGCTGTTA36080.11463193395319726No Hit
GCTGGACACATTGGTC36070.11460016235287764No Hit
CAAAGACCTGCTTTGA35670.11332929834009274No Hit
AATTCTGCTTAGCGAG35490.11275740953433953No Hit
TCGTAGAACCGCACTG34980.11113705791803881No Hit
TCCGTAAGATTACGCA34780.11050162591164636No Hit
TGTCTTCCTGAAATCA34190.10862710149278863No Hit
CCCACCTCTTGACCTA34090.10830938548959243No Hit
ATGGCCGTGGGCCAAA34090.10830938548959243No Hit
GCGTTTAGAAGTCTAC34000.10802344108671583No Hit
CCTGAGGACTGACTCA33200.10548171306114602No Hit
ACAAGACCTAAGCTAA33170.10538639826018717No Hit
TATGGGTACCCTTTGC32870.10443325025059849No Hit
ATAGCCAACATCTGTG32630.10367073184292755No Hit
GCGTTAATGGTACGTT32400.10293998503557625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)