FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4262665
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG415377797.44554169750613No Hit
TAATGGTG120180.28193630041300455No Hit
TATTGGGG118010.27684558838191603No Hit
TATTGGTA98430.23091188258988213No Hit
AATTGGTG85550.20069604343761474No Hit
TATTGGTC76520.17951211272760115No Hit
TATTGGTT66250.15541920371410842No Hit
TATTGGAG63760.1495777876047027No Hit
TTTTGGTG63730.14950740909736046RNA PCR Primer, Index 28 (100% over 8bp)
TATTGCTG62920.14760718939912004No Hit
TATTCGTG58850.1380591719030231No Hit
TATAGGTG53860.12635288018176424RNA PCR Primer, Index 39 (100% over 8bp)
TAGTGGTG42730.100242453957794No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)