FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553942
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT280431.0980280679827499No Hit
TGCTGCTACCGTTCTC261771.024964545005329No Hit
AAACGACCTCTTCAAG251920.9863967153521889No Hit
TATGCTTTGGACTGTT244310.9565996408688999No Hit
ATGGGTTACAATCGAA243020.9515486256148339No Hit
GCCTCTTGATCTGTGA230990.9044449717338923No Hit
AAAGCGGACTCGGGCT223860.8765273447869999No Hit
TCCAAAGTGCTCACGG214730.8407786864384547No Hit
TAGTGCATGAACCCGC200340.7844344155035629No Hit
GCAGGTCTGGCTGTTA199820.7823983473391329No Hit
TAACTCACTGGTGTCC190670.7465713786765714No Hit
TGTGGCTCTTACGATC188200.7369000548955301No Hit
GAAGTATGAACAGGTG186740.7311834019723236No Hit
TTCCCATCTCTTTGTT170960.6693965642132829No Hit
GAGCCTGACCGAGTTT166840.653264639525878No Hit
TCACCTGACTCAATGC162510.6363104565412997No Hit
GGAACTACTACCAAGG161480.6322774753694484No Hit
TGAGAATGAGCAGTTT156470.6126607417083082No Hit
ATGTCCAGAGTCGGTC150990.5912037156677794No Hit
CTAGAGACTAATCTGC149100.5838033909932175No Hit
TCACACAGATTGGGAG145740.5706472582384408No Hit
TTCGTCCGACCTGTGG145470.5695900689992176No Hit
TCGTATACTGCTAATC145450.5695117586852012No Hit
ATTCCTAACTGCTACA142080.5563164707734162No Hit
TCCGCGAGAGAACAGC140360.5495817837679947No Hit
AGGTCGAGAAGGGAGG137720.539244822317813No Hit
ACTGATTCTCGGCTCA137000.536425651013218No Hit
TGGCCTGGACCCGAGT135450.5303566016769371No Hit
TGGACCTCTAGGGAGG133610.5231520527874165No Hit
GCAATCATGGAACGTG129290.5062370249598463No Hit
CCATGATGATTCGGGC127830.5005203720366398No Hit
TTCGATGGAAATCCTG116260.4552178553780783No Hit
ACGAGGATGGAGCTGA115160.4509107881071692No Hit
TGCGGTGACCTTAGTG114370.4478175307035164No Hit
AAGGTGCGAACAGATA110130.4312157441320124No Hit
TCCCAGACTGTCTTGA109330.4280833315713513No Hit
TTTCGTAACAGTACGG109160.42741769390221074No Hit
TGGTCCTTGGAGAGGG107530.4210354033098637No Hit
TCTCTCTACTCTGGAT104940.41089421764472334No Hit
TCCTTCACTTGTCACA94940.37173906063645923No Hit
TTCGATGGACAGGTGA92390.3617544955993519No Hit
AGCCCAGTGAAAGTAC91670.3589353242947569No Hit
GAAGTAATGAGTCTGT91570.35854377272467425No Hit
TCGCCGAGAAGGTAAT91420.3579564453695503No Hit
TGATATCCTCGATGTT90340.3537276884126578No Hit
AGCTGCTCTTGTCACA90050.35259218885941807No Hit
CTTTCGGTGCTTTGTT88420.34620989826707105No Hit
AACTCCCTGCTTCACC86500.33869210812148437No Hit
AACCCTCACGCGGTTC85940.33649941932902155No Hit
ATGGCCGTGATACGCT84050.3290990946544597No Hit
CCCTTGCCTCCTACGG82470.32291257984715394No Hit
TCGGCTTACCTCCTAC82420.3227168040621126No Hit
TGTTTGTTGCAAGTTC78070.3056843107635177No Hit
AAACACGCTGCAGTTT77170.30216034663277397No Hit
CTAACTGGAACCTTGA76500.29953695111322026No Hit
ACCCTTGTGGGCCATC76030.2976966587338319No Hit
TGGCCTTTGCGAAAGC73690.28853435199389804No Hit
TACAACGTGAGCAGGT72710.28469714660708817No Hit
AAATAGGACTCTATCT72170.2825827681286419No Hit
CAATGTGTGGTTGACT71940.2816821995174518No Hit
GGGGGGGGGGGGGGGG70400.2756523053381792No Hit
TCAAAGGCTCTTAGTG70030.2742035645288734No Hit
ACAGCCGACAAACATG69700.2729114443476007No Hit
TGAACTGTGTGCTGGC69160.2707970658691544No Hit
CTTTCGGTGAGCCATT68080.2665683089122619No Hit
GGACATTTGGCCGCTA67330.2636316721366421No Hit
CAGCCGATGTAATAGG62650.24530705865677452No Hit
AGTGTTCGATTCGCCT61670.24146985326996465No Hit
GGCTTGCGAATACGCT60190.23567489003274156No Hit
GATAAGAACATTGGTC56030.21938634471730367No Hit
GTAAAGCCTGAAATCA53940.21120291690257648No Hit
GTGCTAATGGGAGTCA53480.20940177968019635No Hit
CGACGTTCTTGCTACA52760.20658260837560133No Hit
TTGGGAGGAATCTGTG52740.2065042980615848No Hit
TGTACTGGAAATGCTC51170.20035693841128732No Hit
GGACCACTGGCTAGAG50790.1988690424449733No Hit
GGAACATCTTTACGAC49930.19550169894226258No Hit
AGGGACCCTAATCCTG48800.19107716620032875No Hit
TCTTGAACTCGTACAT47400.18559544421917176No Hit
GGGTGAAACCGACTAT47140.18457741013695692No Hit
TACCACCTGAAACATG46640.1826196522865437No Hit
TAACGAGCTTACTTTC46570.18234556618748587No Hit
TACGTGTGAAACGCAG45180.17690299936333714No Hit
AGCTTGTGAACCGTGC45090.17655060295026279No Hit
CGGATGAGAACAGGCA44600.17463200025685785No Hit
CATGATGTGCCTAGTA44040.17243931146439503No Hit
ACTTATCTGTCAGGTT43490.17028577782894053No Hit
AACTAGCACTGCCACG42990.1683280199785273No Hit
TCGGACCGAATGTCCA40140.15716880023117205No Hit
ACGTGTTGATCTTTGT40130.15712964507416377No Hit
AACGAACCTGACACCC40080.15693386928912245No Hit
GTAGATAGAGCTGAGA39390.15423216345555224No Hit
AACTACGACATTGCCA39120.1531749742163291No Hit
GTCCGAGTGGTCTTGA39070.1529791984312878No Hit
TGGTAGGGATGTGCTA39040.152861732960263No Hit
CGTCGTACTTGCGCCA38920.15239187107616384No Hit
TTTAGGGACTGGGCAA36900.1444825293604945No Hit
GGTAGTTTGTCCAGCG36800.14409097779041186No Hit
CTCGGATCTAGTACGG36780.14401266747639532No Hit
CCTTCAGTGCAACGCC36650.1435036504352879No Hit
TACCACCTGTACTTTC36570.14319040917922177No Hit
AGTTTGCGAGCAGGAT36470.14279885760913913No Hit
GCCATAATGACCGTTA36200.141741668369916No Hit
TGAGGAGTGGTCCCAG35600.13939235894942015No Hit
CTTTAACTGTTCGCCT35310.1382568593961805No Hit
CAGGCCATGTCGTAGT32850.12862469077214753No Hit
GAGAAGAGATGCTACA32620.12772412216095747No Hit
CTTACATTGGAAGGCT32540.12741088090489136No Hit
AGAACGCGACACAGTT32480.12717594996284176No Hit
CAAAGACCTGCTTTGA32440.1270193293348087No Hit
GTCCGTTACGGATGGT32370.12674524323575087No Hit
GAGGTTGGAGGAATAT32220.12615791588062691No Hit
GGGATGTGATGCATGT32190.1260404504096021No Hit
TGCAATGACTCCGTAA31670.12400438224517236No Hit
CCGTTACTGTTGGTTG31550.1235345203610732No Hit
CGATCGGTGCGAGGAT31310.12259479659287487No Hit
GAACCATACTTTGGGT31190.1221249347087757No Hit
GAAAGTCCTTATCTGG31070.12165507282467652No Hit
GCTGGACACATTGGTC30370.11891421183409803No Hit
GGCTTAAACTGCATCA30120.11793533290889142No Hit
TCCTCATCTTTCTGGA29960.11730885039675921No Hit
AACTACGACGCTGTTA29550.11570348895942038No Hit
AATTCTGCTTAGCGAG28960.1133933346959328No Hit
TCCGTAAGATTACGCA28920.11323671406789973No Hit
TGTCTTCCTGAAATCA28900.11315840375388322No Hit
TCGTAGAACCGCACTG28580.11190543872961878No Hit
ATGGCCGTGGGCCAAA28190.11037838760629647No Hit
CCTGAGGACTGACTCA27830.10896880195399895No Hit
ATAGCCAACATCTGTG27600.10806823334280889No Hit
CCCACCTCTTGACCTA27550.10787245755776757No Hit
AGGTGATCTGTTAGTC26660.10438764858403207No Hit
ACAAGACCTAAGCTAA26640.10430933827001554No Hit
GGCTTCTACATCAGCT26370.1032521490307924No Hit
TATGGGTACCCTTTGC25840.1011769257093544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)