FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082075
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT330961.0738220192565076No Hit
TGCTGCTACCGTTCTC305860.992383378081325No Hit
AAACGACCTCTTCAAG296650.9625009125345749No Hit
ATGGGTTACAATCGAA292320.9484519357900116No Hit
TATGCTTTGGACTGTT288290.9353763292586975No Hit
GCCTCTTGATCTGTGA273320.8868051556175628No Hit
AAAGCGGACTCGGGCT264370.857766277588962No Hit
TCCAAAGTGCTCACGG254360.8252881581402141No Hit
TAGTGCATGAACCCGC241480.7834981303180487No Hit
GCAGGTCTGGCTGTTA241480.7834981303180487No Hit
GAAGTATGAACAGGTG232560.7545565893107729No Hit
TAACTCACTGGTGTCC226400.7345700542653895No Hit
TGTGGCTCTTACGATC226240.7340509234849899No Hit
TTCCCATCTCTTTGTT202180.6559866323824046No Hit
TCACCTGACTCAATGC198110.6427812431559906No Hit
GAGCCTGACCGAGTTT196640.6380117291110696No Hit
GGAACTACTACCAAGG192210.6236382956287566No Hit
TGAGAATGAGCAGTTT188200.6106275804449923No Hit
CTAGAGACTAATCTGC183430.5951509940543303No Hit
GAAGTAATGAGTCTGT179510.5824322899345409No Hit
ATGTCCAGAGTCGGTC178590.5794472879472433No Hit
TTCGTCCGACCTGTGG173660.5634515707761817No Hit
TCGTATACTGCTAATC173000.5613101563070334No Hit
TGCGGTGACCTTAGTG171360.5559890658079378No Hit
ATTCCTAACTGCTACA170200.552225367650041No Hit
TCACACAGATTGGGAG169160.5488510175774438No Hit
TCCGCGAGAGAACAGC165790.5379168255152779No Hit
AGGTCGAGAAGGGAGG163670.5310383426749836No Hit
ACTGATTCTCGGCTCA161840.5251007843741635No Hit
TGGCCTGGACCCGAGT159730.5182547472076442No Hit
TGGACCTCTAGGGAGG159110.5162431154335958No Hit
CCATGATGATTCGGGC154970.502810606490757No Hit
GCAATCATGGAACGTG148070.48042309158602564No Hit
ACGAGGATGGAGCTGA138690.449989049585101No Hit
TTCGATGGAAATCCTG137210.445187089866405No Hit
AAGGTGCGAACAGATA133290.43246838574661545No Hit
TCCCAGACTGTCTTGA129530.42026881240722563No Hit
TTTCGTAACAGTACGG129090.4188412027611268No Hit
TGGTCCTTGGAGAGGG126260.40965907708280946No Hit
TCTCTCTACTCTGGAT124090.4026183658736403No Hit
GCGGTTCGAGTACGTT122360.39700526431057No Hit
TCCTTCACTTGTCACA111420.3615096972007495No Hit
TTCGATGGACAGGTGA110770.35940072840537624No Hit
TCGCCGAGAAGGTAAT109760.356123715354104No Hit
TGATATCCTCGATGTT108850.35317115904058144No Hit
AGCCCAGTGAAAGTAC107330.34823941662678554No Hit
AGCTGCTCTTGTCACA106070.3441512617311389No Hit
CTTTCGGTGCTTTGTT104530.33915462796979307No Hit
AACTCCCTGCTTCACC103800.33678609378422003No Hit
AACCCTCACGCGGTTC100160.32497586853012983No Hit
GGGGGGGGGGGGGGGG99600.3231589107987314No Hit
CCCTTGCCTCCTACGG99430.32260733434455685No Hit
ATGGCCGTGATACGCT99230.32195842086905735No Hit
TCGGCTTACCTCCTAC94970.30813656384091886No Hit
TGTTTGTTGCAAGTTC92730.3008687329153249No Hit
ACCCTTGTGGGCCATC90840.294736500571855No Hit
AAACACGCTGCAGTTT89350.28990209517938403No Hit
TGGCCTTTGCGAAAGC88850.28827981149063536No Hit
CTAACTGGAACCTTGA87700.2845485590065135No Hit
TACAACGTGAGCAGGT86700.28130399162901615No Hit
AAATAGGACTCTATCT86310.2800386103517922No Hit
CAATGTGTGGTTGACT85840.2785136636843685No Hit
TCAAAGGCTCTTAGTG82010.2660869706285538No Hit
CTTTCGGTGAGCCATT80190.2601818580015087No Hit
TGAACTGTGTGCTGGC78840.25580169204188735No Hit
AGTGTTCGATTCGCCT74360.24126603019069942No Hit
CAGCCGATGTAATAGG74280.2410064648004997No Hit
GAGGTTGGAGGAATAT71570.23221368720748198No Hit
GGCTTGCGAATACGCT68330.2217012889043907No Hit
ACAGCCGACAAACATG68080.2208901470600164No Hit
GATAAGAACATTGGTC67290.21832693883179355No Hit
GTGCTAATGGGAGTCA65980.21407655556727204No Hit
CGACGTTCTTGCTACA65530.21261650024739825No Hit
GTAAAGCCTGAAATCA63410.20573801740710398No Hit
TGTACTGGAAATGCTC61860.20070893797198316No Hit
TTGGGAGGAATCTGTG61190.19853507782906No Hit
GGACATTTGGCCGCTA59490.19301931328731456No Hit
AGGGACCCTAATCCTG58850.19094279016571628No Hit
TAACGAGCTTACTTTC57680.18714664633404443No Hit
GGAACATCTTTACGAC57530.18665996122741985No Hit
GGGTGAAACCGACTAT56940.18474566647469642No Hit
GGACCACTGGCTAGAG56310.18270158902687314No Hit
TCTTGAACTCGTACAT55610.18043039186262502No Hit
CGGATGAGAACAGGCA54910.1781591946983769No Hit
TACGTGTGAAACGCAG54910.1781591946983769No Hit
TACCACCTGAAACATG53800.1745577249093549No Hit
AGCTTGTGAACCGTGC53710.17426571384538014No Hit
ACTTATCTGTCAGGTT51730.16784147043793549No Hit
CATGATGTGCCTAGTA51660.16761435072151068No Hit
AACTAGCACTGCCACG51310.1664787521393866No Hit
ACGTGTTGATCTTTGT49060.15917847554001768No Hit
TGGTAGGGATGTGCTA47650.15460363553774648No Hit
AACTACGACATTGCCA47120.15288401482767291No Hit
TCGGACCGAATGTCCA47060.15268934078502305No Hit
GTAGATAGAGCTGAGA46680.1514564051815741No Hit
GTCCGAGTGGTCTTGA46510.15090482872739958No Hit
CTCGGATCTAGTACGG46040.14937988205997582No Hit
CGTCGTACTTGCGCCA45800.14860118588937646No Hit
AACGAACCTGACACCC45300.14697890220062784No Hit
TTTAGGGACTGGGCAA44390.14402634588710528No Hit
GGTAGTTTGTCCAGCG43790.1420796054606069No Hit
CCTTCAGTGCAACGCC43750.141949822765507No Hit
AGTTTGCGAGCAGGAT42920.13925683184218424No Hit
TACCACCTGTACTTTC42900.1391919404946343No Hit
GCCATAATGACCGTTA42590.13818612460761015No Hit
TGAGGAGTGGTCCCAG41980.13620693850733678No Hit
CTTTAACTGTTCGCCT41870.13585003609581206No Hit
AGAACGCGACACAGTT39150.1270248128290194No Hit
GGGATGTGATGCATGT38970.1264407907010699No Hit
CTTACATTGGAAGGCT38790.12585676857312036No Hit
GTCCGTTACGGATGGT38550.12507807240252103No Hit
GAGAAGAGATGCTACA38440.12472116999099633No Hit
CGATCGGTGCGAGGAT37320.12108725452819934No Hit
CCGTTACTGTTGGTTG36930.1198218732509754No Hit
GAACCATACTTTGGGT36540.11855649197375145No Hit
TGCAATGACTCCGTAA36540.11855649197375145No Hit
CAGGCCATGTCGTAGT36480.1183618179311016No Hit
GAAAGTCCTTATCTGG36450.11826448090977669No Hit
GACGAGTGATGCTACA35710.1158635010504287No Hit
CAAAGACCTGCTTTGA35480.11511725055360432No Hit
AATTCTGCTTAGCGAG35360.11472790246830464No Hit
AACTACGACGCTGTTA35180.11414388034035512No Hit
GCTGGACACATTGGTC35100.11388431495015533No Hit
TCCTCATCTTTCTGGA34960.1134300755173057No Hit
GAAGGACACCCAGCTG34530.11203491154498187No Hit
GGCTTAAACTGCATCA34130.11073708459398296No Hit
TGTCTTCCTGAAATCA33820.10973126870695879No Hit
GCGTTTAGAAGTCTAC33230.10781697395423537No Hit
CCTGAGGACTGACTCA33120.10746007154271067No Hit
CCCACCTCTTGACCTA32890.10671382104588631No Hit
TCGTAGAACCGCACTG32460.10531865707356246No Hit
ATGGCCGTGGGCCAAA32450.10528621139978749No Hit
ACAAGACCTAAGCTAA32390.10509153735713764No Hit
ATAGCCAACATCTGTG32210.10450751522918812No Hit
TATGGGTACCCTTTGC32080.10408572147011348No Hit
TCCGTAAGATTACGCA31720.10291767721421444No Hit
AGGTGATCTGTTAGTC31590.1024958834551398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)