FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048497
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT528891.7349205198496178No Hit
GAAGCAGTGGAACCTT176550.579137850553896No Hit
GTTCTTCCTAACGTAA164510.5396429781626815No Hit
CATGGAGGATCGACAT127160.4171235858195038No Hit
CGCTTAAGATAATAGG125410.411383052041711No Hit
TGTCCCATGAGACAAT120250.394456678159762No Hit
AAAGCATGATCTTTGT116360.3816962916479826No Hit
TGAGCGCACAGGACAC110340.3619488554523754No Hit
TAATTGCCTGTCTGGC105720.3467938462790024No Hit
TGTCGAAACTTCGCAA102850.33737937088342224No Hit
GGAAGGACTAGGTAAT93880.30795503489096426No Hit
GATACGTACCGCTCGA84900.27849789584834755No Hit
AATGATGGAAAGCTAA81160.2662295550889504No Hit
CATGTCAGAGGTTTGT80810.26508144833339187No Hit
TCGACCTTGTTTCGCC77070.25281310757399467No Hit
GGGGGGGGGGGGGGGG75260.24687575549524896No Hit
GTGAGATCTCGATTAC70780.2321799890240994No Hit
GCTATGATGACCTGCG64810.21259656807928626No Hit
ATGGGTACTTGGCTGC62370.2045926238405352No Hit
AAACGACCTAGGGAGG60880.1997049693668716No Hit
AGCCTACCTTAACTCG58450.19173382817827933No Hit
CAGCTCACTGTTTACG57860.1897984482189092No Hit
TGTTCCTCTTTATGGC57390.18825670486144483No Hit
TTGCGATGACATTCAG56700.18599329440048654No Hit
CTGCCTTGACCATAAC53380.17510268174775964No Hit
CAATGTGACCAACGCC53080.17411859024299517No Hit
AGCTTGTGACTCACTC51790.16988699677250788No Hit
TAACCAGACATCACAC51660.16946055712044328No Hit
CATGATGTGTCATGTG51050.1674595710607555No Hit
CCTGTTGACCCTAGTA50000.16401525079407983No Hit
CACAGCTCTGGACTGA48920.16047252137692772No Hit
CATGCAAACGAAGGCT48730.1598492634239102No Hit
ATGGGTACTTTCGGGC47290.1551256242010407No Hit
CTATTCAACCGAACGA47050.15433835099722915No Hit
ATGCCGCGATGCCACG46660.1530590320410353No Hit
TTGTTTGTGATACGCT45800.15023796972737713No Hit
CATTAGTCTATTCTGG45770.15013956057690067No Hit
AGAACGCGAGGAATAT44300.14531751220355474No Hit
ATTAGCGTGGTCGGTC44290.14528470915339592No Hit
CACTATACTACGCCAC43690.14331652614386697No Hit
CCGCTATTGTTCGCAA42640.1398722058771913No Hit
GCAGATACTCGGCTCA41780.1370511435635331No Hit
GCATCTCTGAGCCTAT41660.13665750696162732No Hit
AGCTCGGACCTTAAGC41450.1359686429082922No Hit
ATAGGAAACAGACTTC41110.13485333920289244No Hit
AACCAACACCGGAATG40460.1327211409425694No Hit
GGGATCAGATGAGTAA39950.1310481853844698No Hit
CCCAATGCTGGAATGC39920.13094977623399334No Hit
TGGGCCATGCGTTCTC39890.13085136708351688No Hit
TTGACGTGACCTCAAA39790.13052333658192872No Hit
TTGTAACCTCATCCTC39300.12891598712414673No Hit
CTGATTACTGCTTTGA39040.12806310782001754No Hit
CTGCCATTGCACAGTT39030.12803030476985872No Hit
GGGTCGTACCGCTCGA39020.1279975017196999No Hit
AACGTCCCTTACAGAA38930.12770227426827055No Hit
GATACCATGTCTGGAT38590.12658697056287083No Hit
TCAGGCTCTTAAAGCA38570.1265213644625532No Hit
TGGAGTTACAAGTCTC38390.1259309095596945No Hit
ATAGCCAACATGGCCC38340.12576689430890042No Hit
CCTACGGGATGGAACG38270.12553727295778871No Hit
CGATCAAACCGATCGC37540.12314265029619514No Hit
CGCTTACTGGCCACGT37210.12206014964095421No Hit
TGTTTGTTGCGCTCGA37160.12189613439016014No Hit
ATCTTACTGCGCACTG36870.12094484593555446No Hit
CGCTGAGTGTTAGCTG36700.1203871940828546No Hit
GCGTCGATGCTTGAAC36290.11904226902634316No Hit
TGGGTGCACTTCTGAG35910.11779575312030814No Hit
TCCAAAGTGAAGGCCA35870.11766454091967288No Hit
CTCGGATCTTTCTGAG35820.11750052566887878No Hit
CGCTTCCCTGCGCTGT35640.1169100707660201No Hit
GTAAGAAACGACAAAT35520.11651643416411431No Hit
TTCTAACACTACGTTG35440.1162540097628438No Hit
CATGTTACTAACGTGG34310.1125472650948976No Hit
TACAACCGATTCCATT34210.11221923459330942No Hit
CGAGTCTCTTACTTTC34000.11153037053997429No Hit
CTTAGGCCTTATGACC33750.1107102942860039No Hit
CCTTCAGTGTAGCTTG33690.110513475985051No Hit
GCATAGTACGCTCCTT33480.10982461193171586No Hit
CCTCCCTTGTTTCGCC33410.10959499058060414No Hit
TTCACGCTGTGGGCAA33040.10838127772472796No Hit
TACGCCTTGGGTTGAG33020.10831567162441032No Hit
GGTAGCCTGTGCCACG32960.10811885332345741No Hit
AGTGGTACTCAACGCC32920.10798764112282216No Hit
AGAACAATGAGTCGGA32770.10749559537043991No Hit
GCGACAAGAAAGGCTG32770.10749559537043991No Hit
AACAGCTACTCGAGAG32650.10710195876853412No Hit
ATTGAGAGATGATCAC32620.10700354961805769No Hit
GTGTACTGAATCACAC32590.10690514046758123No Hit
AACCACTGAAAACATG32430.10638029166504018No Hit
AGCCGGTACGCGCAAA32320.10601945811329322No Hit
TATCTCCCTGGAATTA32260.10582263981234032No Hit
ATCGCAATGTAACTCG32220.10569142761170504No Hit
TGAGATAGACTCATCC32170.10552741236091097No Hit
GCATATCGAGCCGCTA32110.10533059405995807No Hit
GGTCAGAGAACCAGTT31880.1045761239063053No Hit
ACCTCCATGGAATTGA31780.10424809340471713No Hit
CATGTCAGAACAGGTG31250.1025095317462999No Hit
AGAACATGAAAGCTAA31070.10191907684344122No Hit
GGGCTGACTAATACAG30920.10142703109105897No Hit
TTCTATGACCAACGCC30870.10126301584026488No Hit
AGGTTACTGGTCCCGA30830.10113180363962963No Hit
CGGTTCACTGGAATGC30750.1008693792383591No Hit
GAAAGTAACCCTGCGA30620.1004429395862945No Hit
ATTCCTAACATTGCCA30560.10024612128534159No Hit
CCTTCTTTGGATCATC30520.10011490908470634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)