FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181594
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT587571.8467786901785708No Hit
GAAGCAGTGGAACCTT182590.5738947206966067No Hit
GTTCTTCCTAACGTAA159080.5000009429235786No Hit
CGCTTAAGATAATAGG136510.42906165903003335No Hit
CATGGAGGATCGACAT133000.41802945316089984No Hit
TGTCCCATGAGACAAT131680.4138805894152428No Hit
AAAGCATGATCTTTGT124170.39027606916533036No Hit
TGAGCGCACAGGACAC116780.3670487183468412No Hit
GATACGTACCGCTCGA114840.3609511458721635No Hit
TGTCGAAACTTCGCAA110160.3462415380466521No Hit
TAATTGCCTGTCTGGC108010.3394839190669834No Hit
GGGGGGGGGGGGGGGG103410.3250257575290876No Hit
CATGTCAGAGGTTTGT88900.279419687112812No Hit
GGAAGGACTAGGTAAT88260.2774081168118874No Hit
AATGATGGAAAGCTAA85590.26901609696271744No Hit
TCGACCTTGTTTCGCC81310.255563720575284No Hit
ATGGGTACTTGGCTGC68580.21555233005845498No Hit
GTGAGATCTCGATTAC67090.21086914295161482No Hit
AAACGACCTAGGGAGG62720.19713388949061383No Hit
TTGCGATGACATTCAG61270.19257642552758145No Hit
AGCCTACCTTAACTCG59770.18786180763478935No Hit
TGTTCCTCTTTATGGC58210.18295860502628558No Hit
GCTATGATGACCTGCG57170.17968980328728304No Hit
CCTGTTGACCCTAGTA57010.1791869107120519No Hit
CAGCTCACTGTTTACG56010.17604383211685715No Hit
CTGCCTTGACCATAAC54550.17145493736787282No Hit
CAATGTGACCAACGCC54540.1714235065819209No Hit
CACAGCTCTGGACTGA54380.17092061400668973No Hit
TAACCAGACATCACAC53530.1682489972007742No Hit
CATGCAAACGAAGGCT53120.16696033497674437No Hit
CATGATGTGTCATGTG52700.16564024196676258No Hit
ATGCCGCGATGCCACG51840.1629371943748951No Hit
ATGGGTACTTTCGGGC51160.1607999009301627No Hit
CTATTCAACCGAACGA50810.15969982342184452No Hit
TTGTTTGTGATACGCT50070.15737394526140042No Hit
TGGGCCATGCGTTCTC49670.1561167138233225No Hit
CATTAGTCTATTCTGG49190.15460803609762905No Hit
AGAACGCGAGGAATAT49000.15401085116454205No Hit
ATTAGCGTGGTCGGTC48990.1539794203785901No Hit
CCGCTATTGTTCGCAA46270.14543024659966042No Hit
CACTATACTACGCCAC45160.14194142935899426No Hit
AACCAACACCGGAATG44040.13842118133237616No Hit
AGCTTGTGACTCACTC43880.137918288757145No Hit
ATAGGAAACAGACTTC43780.13760398089762552No Hit
CTGCCATTGCACAGTT43590.13700679596453852No Hit
AACGTCCCTTACAGAA43100.13546668745289311No Hit
GGGCTGACTAATACAG42620.13395800972719965No Hit
AGCTCGGACCTTAAGC42540.13370656343958406No Hit
TTGACGTGACCTCAAA42190.1326064859312659No Hit
CCCAATGCTGGAATGC41930.13178928549651528No Hit
GGTAGCCTGTGCCACG41520.1305006232724854No Hit
CTGATTACTGCTTTGA41310.12984057676749455No Hit
ATAGCCAACATGGCCC41190.12946340733607117No Hit
TTGTAACCTCATCCTC41160.12936911497821532No Hit
CCTACGGGATGGAACG41090.12914909947655168No Hit
CGCTTACTGGCCACGT41090.12914909947655168No Hit
TGGAGTTACAAGTCTC41080.12911766869059974No Hit
TGTTTGTTGCGCTCGA40990.1288347916170322No Hit
GCAGATACTCGGCTCA40670.12782900646656992No Hit
CGCTGAGTGTTAGCTG40330.1267603597442037No Hit
CGATCAAACCGATCGC39780.12503166651684658No Hit
GCATCTCTGAGCCTAT39430.12393158900852842No Hit
TCAGGCTCTTAAAGCA39110.12292580385806612No Hit
ATCTTACTGCGCACTG38930.12236004971093106No Hit
CATGTTACTAACGTGG38770.12185715713569992No Hit
GGGATCAGATGAGTAA38550.12116567984475707No Hit
CGCTTCCCTGCGCTGT37950.11927983268764021No Hit
TCCAAAGTGAAGGCCA37810.11883980168431296No Hit
TGGGTGCACTTCTGAG37550.11802260124956232No Hit
AGCCGGTACGCGCAAA37480.1178025857478987No Hit
TTCTAACACTACGTTG36740.1154767075874546No Hit
CGAGTCTCTTACTTTC36720.11541384601555069No Hit
CTCGGATCTTTCTGAG36280.114030891433665No Hit
AGTGGTACTCAACGCC36000.11315082942701049No Hit
TACAACCGATTCCATT35940.1129622447112988No Hit
CTTAGGCCTTATGACC35810.1125536444939235No Hit
GATACCATGTCTGGAT35580.1118307364170287No Hit
CCTTCAGTGTAGCTTG35440.11139070541370143No Hit
CCTCCCTTGTTTCGCC35370.11117068991203781No Hit
AGAACAATGAGTCGGA34960.10988202768800795No Hit
ACCTGCTTGCCAAACT34890.10966201218634433No Hit
TGAGATAGACTCATCC34790.10934770432682486No Hit
TACGCCTTGGGTTGAG34760.10925341196896901No Hit
ATCGCAATGTAACTCG34650.1089076733234976No Hit
AACAGCTACTCGAGAG34570.10865622703588201No Hit
ATTGAGAGATGATCAC34540.10856193467802618No Hit
CATGTCAGAACAGGTG34360.10799618053089113No Hit
AACCACTGAAAACATG34140.10730470323994827No Hit
TATCTCCCTGGAATTA34110.10721041088209245No Hit
GTGTACTGAATCACAC34100.10717898009614049No Hit
TTCTATGACCAACGCC33410.10501025586545612No Hit
TTCACGCTGTGGGCAA33390.10494739429355222No Hit
ACCTCCATGGAATTGA33270.10457022486212886No Hit
AGGTTACTGGTCCCGA33170.10425591700260939No Hit
CGGTTCACTGGAATGC33160.10422448621665743No Hit
TGGTCCTTGTTTCTGT33050.10387874757118601No Hit
GCATGGGCTATGTGGG32980.10365873206952239No Hit
CCTTCTTTGGATCATC32910.10343871656785875No Hit
AGAACATGAAAGCTAA32890.10337585499595486No Hit
GTTGCTAGACGCTCGA32870.10331299342405097No Hit
AGAGAATTGCCCGCAA32820.10315583949429123No Hit
ATTCCTAACGGACAGT32810.10312440870833928No Hit
TCCGGGATGCGAGTTT32760.10296725477857954No Hit
ATTCCTAACATTGCCA32210.10123856155122243No Hit
TATCATGCTTGTGGGA32050.10073566897599129No Hit
TCGACCTTGGGACCTC32030.10067280740408738No Hit
GTCCTGACTTTACGAC31910.10029563797266403No Hit
AAAGGATACTGCTGGC31910.10029563797266403No Hit
TTCGACCACAGTACGG31880.10020134561480817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)