FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2655355
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG255590696.25477572678605No Hit
GGACAGGA306121.1528402040405143No Hit
GGCCAGGG258770.9745212975289557ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGC48660.18325233349213194No Hit
GGACACGG42710.16084478346586426No Hit
GGAAAGGG34480.1298508109085226No Hit
GGACAGCG32050.12069949215829899No Hit
CGACAGGG31670.11926842173645331No Hit
GCACAGGG28400.10695368415899194No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)