FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15732758
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC2901.9917934E-99.1717633
TCTATAC13100.08.9764633
TATACTG18900.08.5179965
TAGACTG19450.08.2771265
CTAGGAC20850.08.2584083
TACACCG7250.08.1098465
TAGGACT21100.08.0942644
CTTATAC15650.08.0546681
TAGACCG4709.094947E-127.74420555
TACACTG24300.07.71969035
TATACCG3454.426147E-87.70967155
TATACAC30600.07.5942323
TAGGACA19550.07.5903034
CTAGTAC9000.07.4661373
ATACCGT4001.0251824E-87.34954836
TGTACCG4801.2369128E-107.29121885
GTCTAGG21600.07.262461
TAGACAG27800.07.25135335
GTATATA28600.07.247951
TAGTACT11800.07.236824
Produced by FastQC (version 0.11.5)