FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18088002
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263780.14583147436626778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG14600.08.6344061
TACACTG34200.08.43055
TCTATAC16050.08.3718543
CTAGACA30150.08.1238034
TATACTG21800.08.0253835
AGGACCG19200.08.0186945
TAGTACT16750.07.93811564
GTCTTAG21200.07.9284481
TAGACAG30250.07.86567075
GTGTAGG29800.07.80251551
TCTAGAC22300.07.6573843
TAGACCG6400.07.6542095
GTAGTAC13000.07.64434773
CTAGGAC27850.07.6391293
ACACTGT47850.07.57624636
GACCGTA4656.002665E-117.52570777
GTGTATA34850.07.5159111
ATACCGT5600.07.4984156
GTGTAGA26350.07.49516731
TATACCG2851.0131005E-57.3664565
Produced by FastQC (version 0.11.5)