FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18088002
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC17950.011.77703822
CTTATAC24450.010.1357061
CCCACGA23250.09.75454919
ATCTCCG22300.09.54266210
TATACAC38000.09.54219153
CACGAGA23900.09.3136621
TCTCCGA24400.09.00825411
TAGACCG6550.08.763565
TAGACAG32550.08.5163255
TATACTG24250.08.4290595
TACACTG31000.08.2647065
TAGACTG25550.08.2193665
CTAGGAC28100.08.0214253
AGGACTG46950.07.90220455
TACACAT42750.07.8598295
GACCAAC27800.07.856099626
GTGTAGG29600.07.85192161
TTATACA34950.07.81112
CCACGAG29100.07.745475320
CTAATAC19850.07.6877273
Produced by FastQC (version 0.11.5)