FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3787376
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT403141.0644308882983893No Hit
AAACGACCTCTTCAAG373650.9865669529510669No Hit
TGCTGCTACCGTTCTC365310.9645464300349371No Hit
ATGGGTTACAATCGAA363220.9590280975535569No Hit
TATGCTTTGGACTGTT356760.9419714335201997No Hit
GCCTCTTGATCTGTGA329610.8702859182716478No Hit
AAAGCGGACTCGGGCT326850.8629985509756624No Hit
TCCAAAGTGCTCACGG316850.836595046280063No Hit
TAGTGCATGAACCCGC288170.7608697948130844No Hit
GCAGGTCTGGCTGTTA285680.7542953221438801No Hit
GAAGTATGAACAGGTG279010.7366841845119153No Hit
TGTGGCTCTTACGATC276350.729660852262886No Hit
TAACTCACTGGTGTCC276180.7292119926830608No Hit
TTCCCATCTCTTTGTT252560.6668469145920554No Hit
TCACCTGACTCAATGC237520.627136043529874No Hit
TGAGAATGAGCAGTTT234630.6195054306728458No Hit
GAGCCTGACCGAGTTT227040.5994651706088859No Hit
GGAACTACTACCAAGG225540.5955046449045461No Hit
CTAGAGACTAATCTGC220990.5834910502680484No Hit
TCGTATACTGCTAATC219820.5804018402186633No Hit
ATGTCCAGAGTCGGTC214890.5673849124037328No Hit
GAAGTAATGAGTCTGT213850.5646389479153905No Hit
TTCGTCCGACCTGTGG211530.5585133348260114No Hit
TGCGGTGACCTTAGTG210450.5556617563188868No Hit
ATTCCTAACTGCTACA210080.5546848266451495No Hit
TCACACAGATTGGGAG209660.5535758794479344No Hit
AGGTCGAGAAGGGAGG207510.5478991259383806No Hit
ACTGATTCTCGGCTCA201430.5318457950834562No Hit
TCCGCGAGAGAACAGC200280.5288093920434622No Hit
TGGACCTCTAGGGAGG196180.5179839551182666No Hit
TGGCCTGGACCCGAGT194140.5125976401603644No Hit
CCATGATGATTCGGGC188630.49804930907308914No Hit
TTCGATGGAAATCCTG173660.458523262543777No Hit
GCAATCATGGAACGTG170970.4514207197806608No Hit
ACGAGGATGGAGCTGA168390.44460861556919623No Hit
AAGGTGCGAACAGATA166680.4400936162662487No Hit
TTTCGTAACAGTACGG164060.4331758980360017No Hit
TGGTCCTTGGAGAGGG160870.4247531800381055No Hit
TCCCAGACTGTCTTGA160190.4229577417188048No Hit
TCTCTCTACTCTGGAT155010.4092807262864844No Hit
GCGGTTCGAGTACGTT143360.37852064331611124No Hit
TTCGATGGACAGGTGA138080.3645795928368348No Hit
TCGCCGAGAAGGTAAT135690.3582691552145866No Hit
TGATATCCTCGATGTT135420.3575562605878054No Hit
TCCTTCACTTGTCACA134560.35528555918398386No Hit
AGCCCAGTGAAAGTAC134190.35430862951024666No Hit
AGCTGCTCTTGTCACA129770.34263828043479183No Hit
AACTCCCTGCTTCACC128900.3403411755262747No Hit
CTTTCGGTGCTTTGTT128250.3386249477210607No Hit
AACCCTCACGCGGTTC124400.32845959841325495No Hit
CCCTTGCCTCCTACGG122430.3232581079882219No Hit
ATGGCCGTGATACGCT122360.32307328345535274No Hit
TCGGCTTACCTCCTAC121050.31961442434022924No Hit
GGGGGGGGGGGGGGGG116920.3087097769009467No Hit
TGTTTGTTGCAAGTTC112150.29611530516114587No Hit
AAACACGCTGCAGTTT111210.29363337571975956No Hit
ACCCTTGTGGGCCATC109990.2904121481468964No Hit
TGGCCTTTGCGAAAGC108300.28594995585334015No Hit
CTAACTGGAACCTTGA108060.2853162717406458No Hit
AAATAGGACTCTATCT105370.27821372897752955No Hit
CAATGTGTGGTTGACT104100.27486048388118844No Hit
TACAACGTGAGCAGGT103820.27412118574971167No Hit
TCAAAGGCTCTTAGTG101740.268629256773027No Hit
CTTTCGGTGAGCCATT97830.2583054864370477No Hit
TGAACTGTGTGCTGGC96800.255585925453401No Hit
AGTGTTCGATTCGCCT90310.23845005090595706No Hit
GAGGTTGGAGGAATAT85930.22688531584928456No Hit
CAGCCGATGTAATAGG85320.225274702062853No Hit
GTGCTAATGGGAGTCA79680.21038312541453502No Hit
GGCTTGCGAATACGCT79340.20948540625488465No Hit
ACAGCCGACAAACATG78120.20626417868202154No Hit
TTGGGAGGAATCTGTG77420.20441593335332958No Hit
TGTACTGGAAATGCTC77370.20428391582985161No Hit
GATAAGAACATTGGTC77360.20425751232515602No Hit
CGACGTTCTTGCTACA77320.2041518983063736No Hit
GTAAAGCCTGAAATCA74640.197075759047953No Hit
AGGGACCCTAATCCTG73180.19322084736239548No Hit
TAACGAGCTTACTTTC69760.18419084875650055No Hit
TACGTGTGAAACGCAG68890.1818937438479834No Hit
GGGTGAAACCGACTAT68610.18115444571650663No Hit
TACCACCTGAAACATG68100.17980786697703108No Hit
TCTTGAACTCGTACAT67940.1793854109019015No Hit
CGGATGAGAACAGGCA67910.17930620038781467No Hit
AGCTTGTGAACCGTGC66150.1746591835613892No Hit
GGACATTTGGCCGCTA65960.17415751697217283No Hit
GGAACATCTTTACGAC65860.1738934819252168No Hit
GGACCACTGGCTAGAG65320.17246769267165446No Hit
CATGATGTGCCTAGTA65290.17238848215756766No Hit
ACTTATCTGTCAGGTT65000.1716227805213953No Hit
AACTAGCACTGCCACG61540.16248716789671794No Hit
ACGTGTTGATCTTTGT59590.15733848448107607No Hit
TGGTAGGGATGTGCTA58640.15483015153499416No Hit
CGTCGTACTTGCGCCA57380.15150330994334865No Hit
AACTACGACATTGCCA57100.15076401181187185No Hit
TCGGACCGAATGTCCA56210.14841409989396354No Hit
CTCGGATCTAGTACGG55100.14548331087275201No Hit
AACGAACCTGACACCC54940.14506085479762243No Hit
GTCCGAGTGGTCTTGA54240.14321260946893047No Hit
CCTTCAGTGCAACGCC53940.1424205043280625No Hit
TACCACCTGTACTTTC53350.14086269755102213No Hit
TTTAGGGACTGGGCAA53290.14070427652284856No Hit
GTAGATAGAGCTGAGA53020.13999138189606736No Hit
AGTTTGCGAGCAGGAT51960.13719261039833383No Hit
TGAGGAGTGGTCCCAG51540.13608366320111867No Hit
GGTAGTTTGTCCAGCG50610.13362813726442793No Hit
CTTTAACTGTTCGCCT49710.131251821841824No Hit
AGAACGCGACACAGTT49060.12953559403661005No Hit
GCCATAATGACCGTTA48490.12803059426896088No Hit
CTTACATTGGAAGGCT47470.12533743679000975No Hit
GTCCGTTACGGATGGT46050.12158813912323467No Hit
CCGTTACTGTTGGTTG45660.1205584024401063No Hit
GGGATGTGATGCATGT45550.12026796388845469No Hit
TGCAATGACTCCGTAA44760.11818208701750235No Hit
TCCTCATCTTTCTGGA44740.11812928000811117No Hit
CAGGCCATGTCGTAGT44730.11810287650341555No Hit
AACTACGACGCTGTTA44290.11694112229680918No Hit
GAGAAGAGATGCTACA44220.11675629776394No Hit
GAACCATACTTTGGGT43390.11456480687420525No Hit
GAAAGTCCTTATCTGG43360.11448559636011844No Hit
AATTCTGCTTAGCGAG41750.11023463210412697No Hit
CCCACCTCTTGACCTA41270.1089672638787382No Hit
ATAGCCAACATCTGTG41030.10833357976604382No Hit
CAAAGACCTGCTTTGA40840.10783191317682743No Hit
CGATCGGTGCGAGGAT40800.10772629915804505No Hit
TGTCTTCCTGAAATCA40450.10680217649369907No Hit
GGCTTAAACTGCATCA40340.10651173794204748No Hit
GAAGGACACCCAGCTG40270.10632691340917827No Hit
CCTGAGGACTGACTCA40120.1059308608387443No Hit
GCTGGACACATTGGTC40110.1059044573340487No Hit
ATGGCCGTGGGCCAAA40010.1056404222870927No Hit
ACAAGACCTAAGCTAA39700.10482191364152911No Hit
TCGTAGAACCGCACTG39170.10342252789266235No Hit
TATGGGTACCCTTTGC38880.10265682625648997No Hit
AGGTGATCTGTTAGTC38840.10255121223770758No Hit
TCCGTAAGATTACGCA38560.10181191410623079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)