FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329612
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC151180.6489492670882533No Hit
CTCCTAGTGAACGTAA119380.5124458493517375No Hit
TAGCTTTACCCTATCC106910.45891762233367617No Hit
CGACCAGACGCGCTGT92040.3950872505807834No Hit
CCAGGACTGTAACTCG83290.35752734790171065No Hit
ATCTGTACTGGGACTA77720.3336177869962895No Hit
GTTCTCGACCGTAGAG73740.3165333969776941No Hit
CTTCCTTTGGCTCAGC71600.3073473179224695No Hit
CGATCAAACCCGGCTA69830.2997494861805314No Hit
TGCTGCTACTTTCTTG60370.259141865684071No Hit
GGGGGGGGGGGGGGGG55310.23742151053480148No Hit
ACAGCCGACTTGCGTA54600.2343737927174139No Hit
GGCAACAGAATAGCAA52820.22673303537241396No Hit
AACTTACCTCACCTAA51360.22046589732539154No Hit
CGCGTTGGAGCGGTTC48060.20630044831499839No Hit
NNNNNNNNNNNNNNNN47060.2020078880088186No Hit
ATCGAAAGATTTCCGC45610.19578367556485804No Hit
GTAACTGTGTGAAAGT44360.19041797518213333No Hit
CCCTGAACTAGCTTTG44210.18977409113620636No Hit
AGTGGAGTGAGTTGAA43880.18835754623516707No Hit
CAATTCGGATAACGGG42270.18144652414221765No Hit
GGGTAGAACCAGTCTA40700.17470720446151547No Hit
AAGAACCCTTAACCCA39010.16745277754407173No Hit
TTCCTAAACTAATGCC38080.16346069645932457No Hit
GTGAAGCGATCCCAGG37300.16011249942050437No Hit
TTTAGTCTGAGCAATA37020.15891058253477405No Hit
CGCGATCGATATCTGG36520.15676430238168415No Hit
TCCGGGAACGTTCTCC36500.15667845117556056No Hit
TGGAACACTCCTAGTA35860.15393121257960554No Hit
CACATCCTGAGCCTAT34940.14998205709792017No Hit
TGCAGATACTGCCACG34920.14989620589179659No Hit
CTTTAACTGTCCGTAA33810.14513146395193707No Hit
TGATAGAGAGACTGTT32910.1412681596763753No Hit
GCAACTTGAAGGTGCA32610.13998039158452136No Hit
CATAGGTACAAGGTGC32490.1394652843477798No Hit
AGTCCTGCTCGAACGA32420.13916480512634724No Hit
TCTCAGAGATGCATGT31950.13714730178244275No Hit
TGCAGTATGACACCGC31910.13697559937019554No Hit
TACGTTTACTGCACCG31670.1359453848967124No Hit
AGGTGTGGATCATGTG31520.13530150085078546No Hit
CTATTGGGACTTGTGA31350.1345717655987349No Hit
ATTTCTGACGGGTAGT31200.13392788155280794No Hit
TGAACTGTGAACGTAA30750.13199622941502706No Hit
GAGTGTTACACCTGCG30470.1307943125292967No Hit
TCAAAGCACTCTGCAA30460.13075138692623495No Hit
TGGCAATTGAGTGGTG30200.1296353212466282No Hit
TGAGGGATGTACGTTG30090.12916313961294842No Hit
CGAGTCTCTGCTACGC29970.12864803237620687No Hit
GGTCGCATGGTACTAC29490.12658760342924058No Hit
CTTAGATCTTGTCACA29420.126287124207808No Hit
TCTATGGGATGCTCAA29110.12495643051289226No Hit
ATGTACGTGTACTTTC28880.12396914164247094No Hit
TTCTTAGACGTCCCGA28870.12392621603940913No Hit
AGAGTACCTTAATCGC28790.12358281121491477No Hit
GGTCTAAACTCCTACC28150.12083557261895973No Hit
GGGTGTGCTCGCAAAG27940.11993413495466197No Hit
GCATGATACTTGCGTA27870.11963365573322939No Hit
GGGATGTGACTGGAAG27240.11692934274033617No Hit
GTAATCTCTACACAAA27190.11671471472502717No Hit
TCCATTTGAAATCTGC26880.11538402103011146No Hit
CGAGTCTCTTGGAACG26430.11345236889233057No Hit
CACCAGGTGCGCCACA26360.11315188967089798No Hit
AGACTACTGGCCCTAG26300.1128943360525272No Hit
TACTGTTTGCTTAAGC26240.11263678243415642No Hit
CTACAACGAATCTGTG26180.11237922881578563No Hit
TCCGGGTGAACACCTA25830.11087683270862272No Hit
CTAGCTCACGAATAGT25770.11061927909025195No Hit
GGCTGACTGAAACTGA25750.11053342788412836No Hit
AGCCAATTGTTATGGC25710.11036172547188114No Hit
TTGCGGTCTTTTGGGT25660.11014709745657217No Hit
CATTCTATGCCTAGCG25600.10988954383820138No Hit
CTCAATTTGGAATCTC25450.10924565979227442No Hit
TCGGACCGATAGGCGA25360.10885932936471825No Hit
CATAGTCGAAGATTCC24780.10636964438713399No Hit
CACATTACTGATGGAC24710.1060691651657014No Hit
CTTTCGTCTCGAACGA24580.10551113232589804No Hit
TGTAAGCACGCGCTGT24480.10508187629528008No Hit
TGCTAGTGAAGGCGTA24190.10383703380648796No Hit
ACCAAGTTGAAGCTAA24180.10379410820342615No Hit
TCGTTGCTGTATTGCG24060.10327900096668459No Hit
AGGCGATGACCTGCGA24010.10306437295137559No Hit
TGCGCAGACATCAGGG23900.10259219131769583No Hit
ATGTCCAGACCGCTAG23730.10186245606564526No Hit
ATAGCCAACCGTTCTC23490.10083224159216213No Hit
TAGTACCGAACGAGCA23300.10001665513398798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)