FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491016
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT433691.7410165169553307No Hit
GAAGCAGTGGAACCTT160700.6451182971125036No Hit
GTTCTTCCTAACGTAA129820.5211528147551039No Hit
GATACGTACCGCTCGA111850.4490135751837804No Hit
CGCTTAAGATAATAGG102010.40951162096108573No Hit
CATGGAGGATCGACAT97020.38947963401278834No Hit
TGTCCCATGAGACAAT96660.38803444056561665No Hit
AAAGCATGATCTTTGT92000.36932721427722665No Hit
TGAGCGCACAGGACAC89160.35792624374953835No Hit
TAATTGCCTGTCTGGC83760.336248342041962No Hit
TGTCGAAACTTCGCAA82810.3324346371119254No Hit
GGAAGGACTAGGTAAT73600.2954617714217813No Hit
GGGGGGGGGGGGGGGG73240.29401657797460956No Hit
AATGATGGAAAGCTAA65590.26330621722220976No Hit
CATGTCAGAGGTTTGT65030.26105813852660925No Hit
TCGACCTTGTTTCGCC62040.24905500406259937No Hit
GTGAGATCTCGATTAC53290.2139287744438414No Hit
ATGGGTACTTGGCTGC51730.2076662695060971No Hit
AAACGACCTAGGGAGG47710.19152827601267916No Hit
CAGCTCACTGTTTACG46290.18582779074883501No Hit
TTGCGATGACATTCAG46210.1855066366494635No Hit
AGCCTACCTTAACTCG45210.18149221040731975No Hit
GCTATGATGACCTGCG45140.18121120057036968No Hit
TGTTCCTCTTTATGGC44550.17884268908750486No Hit
CAATGTGACCAACGCC41800.1678030169216095No Hit
CCTGTTGACCCTAGTA41690.16736143003497367No Hit
CTGCCTTGACCATAAC41080.164912630027266No Hit
TAACCAGACATCACAC40760.16362801362977997No Hit
AGCTTGTGACTCACTC40080.1608982037851222No Hit
CATGCAAACGAAGGCT39680.1592924332882647No Hit
ATGCCGCGATGCCACG39670.1592522890258433No Hit
CACAGCTCTGGACTGA39630.15909171197615754No Hit
CATGATGTGTCATGTG39300.1577669513162501No Hit
CTATTCAACCGAACGA38240.15351165949957768No Hit
ATGGGTACTTTCGGGC37610.1509825709670271No Hit
TGGGCCATGCGTTCTC37430.15025997424344123No Hit
AGAACGCGAGGAATAT36810.1477710299733121No Hit
ATTAGCGTGGTCGGTC36650.1471287217745691No Hit
TTGTTTGTGATACGCT36110.14496093160381146No Hit
CCGCTATTGTTCGCAA36060.14476021029170427No Hit
CATTAGTCTATTCTGG35940.144278479142647No Hit
CACTATACTACGCCAC34770.13958160043933882No Hit
GGGCTGACTAATACAG34450.1382969840418528No Hit
GGGTCGTACCGCTCGA34000.13649049223288812No Hit
AACGTCCCTTACAGAA33980.13641020370804524No Hit
CCCAATGCTGGAATGC33660.1351255873105592No Hit
AGCTCGGACCTTAAGC33570.13476428894876627No Hit
AACCAACACCGGAATG32970.13235563320348004No Hit
ATAGGAAACAGACTTC32750.13147245943020838No Hit
TTGTAACCTCATCCTC32570.1307498627066225No Hit
TTGACGTGACCTCAAA32540.1306294299193582No Hit
GGTAGCCTGTGCCACG32380.12998712172061522No Hit
CTGATTACTGCTTTGA32240.12942510204671506No Hit
ATAGCCAACATGGCCC32160.12910394794734356No Hit
CTGCCATTGCACAGTT31760.12749817745048608No Hit
TGGAGTTACAAGTCTC31630.12697630203900737No Hit
GGCTTGCGAGTACGTT31330.12577197416636424No Hit
CCTACGGGATGGAACG31170.12512966596762123No Hit
TGTTTGTTGCGCTCGA30350.12183783644906336No Hit
CGATCAAACCGATCGC30190.12119552825032036No Hit
TCAGGCTCTTAAAGCA29920.12011163316494153No Hit
ATCTTACTGCGCACTG29870.11991091185283434No Hit
CGCTTACTGGCCACGT29680.11914817086682702No Hit
CGCTGAGTGTTAGCTG29560.11866643971776977No Hit
TGGGTGCACTTCTGAG29440.11818470856871253No Hit
TCCAAAGTGAAGGCCA29420.11810442004386965No Hit
GCAGATACTCGGCTCA29000.11641836102216926No Hit
GATACCATGTCTGGAT28970.11629792823490495No Hit
CATGTTACTAACGTGG28870.11589648561069057No Hit
GCGTCGATGCTTGAAC28620.11489287905015463No Hit
CTCGGATCTTTCTGAG28510.11445129216351882No Hit
GTAAGAAACGACAAAT28280.11352797412782577No Hit
CGCTTCCCTGCGCTGT28140.11296595445392563No Hit
TTCTAACACTACGTTG28040.11256451182971125No Hit
CGAGTCTCTTACTTTC27990.11236379051760408No Hit
AGCCGGTACGCGCAAA27680.1111193183825395No Hit
AGTGGTACTCAACGCC27500.11039672165895362No Hit
ACCTGCTTGCCAAACT27310.1096339806729463No Hit
CCTTCAGTGTAGCTTG27210.10923253804873193No Hit
CTTAGGCCTTATGACC27120.10887123968693899No Hit
AACAGCTACTCGAGAG27010.10842965280030317No Hit
CCTCCCTTGTTTCGCC26960.10822893148819598No Hit
GCGACGTTGCTTAGTG26860.1078274888639816No Hit
AGAACAATGAGTCGGA26810.10762676755187442No Hit
GAAAGTAACCCTGCGA26810.10762676755187442No Hit
ATTGAGAGATGATCAC26800.10758662328945298No Hit
TACAACCGATTCCATT26720.10726546919008148No Hit
GTCCTGACTTTACGAC26630.10690417082828854No Hit
AACCACTGAAAACATG26560.10662316099133848No Hit
ATCGCAATGTAACTCG26470.10626186262954554No Hit
TTCACGCTGTGGGCAA26410.1060209970550169No Hit
TATCTCCCTGGAATTA26370.10586042000533116No Hit
GCGACAAGAAAGGCTG26300.1055794101683811No Hit
AGAACATGAAAGCTAA26210.10521811180658816No Hit
TGAGATAGACTCATCC25960.10421450524605222No Hit
GGTCAGAGAACCAGTT25840.10373277409699497No Hit
TACGCCTTGGGTTGAG25700.10317075442309483No Hit
ACCTCCATGGAATTGA25580.10268902327403757No Hit
TCCGGGATGCGAGTTT25580.10268902327403757No Hit
GTTGCTAGACGCTCGA25470.10224743638740177No Hit
AGGTTACTGGTCCCGA25400.1019664265504517No Hit
ATTCCTAACATTGCCA25300.10156498392623733No Hit
GTGTACTGAATCACAC25130.1008825314650729No Hit
AGAGAATTGCCCGCAA25110.10080224294023No Hit
TGGTCCTTGTTTCTGT25020.10044094457843708No Hit
CATGTCAGAACAGGTG24980.10028036752875132No Hit
TTCTATGACCAACGCC24960.10020007900390844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)