FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189612
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT330361.0357372620870502No Hit
TGCTGCTACCGTTCTC324611.0177099910584737No Hit
AAACGACCTCTTCAAG317790.9963280800297967No Hit
ATGGGTTACAATCGAA306840.9619978856362467No Hit
TATGCTTTGGACTGTT300700.942747895355297No Hit
AAAGCGGACTCGGGCT281400.8822389682506837No Hit
GCCTCTTGATCTGTGA273530.8575651207733104No Hit
GCAGGTCTGGCTGTTA271620.8515769316142529No Hit
TCCAAAGTGCTCACGG265650.8328599215202351No Hit
TAGTGCATGAACCCGC251340.7879955304908559No Hit
TGTGGCTCTTACGATC238380.7473636291812296No Hit
TAACTCACTGGTGTCC236550.7416262542277869No Hit
GAAGTATGAACAGGTG234390.7348542706761826No Hit
TTCCCATCTCTTTGTT216500.6787659439455332No Hit
TCACCTGACTCAATGC207920.6518661203933268No Hit
TGAGAATGAGCAGTTT198830.6233673562803249No Hit
GGAACTACTACCAAGG197010.6176613331025842No Hit
GAGCCTGACCGAGTTT196030.6145888590838008No Hit
ATGTCCAGAGTCGGTC189830.5951507581486399No Hit
CTAGAGACTAATCTGC188050.5895701420737067No Hit
TTCGTCCGACCTGTGG185290.5809170519799901No Hit
TCGTATACTGCTAATC184580.5786910759051571No Hit
GAAGTAATGAGTCTGT183050.5738942542227706No Hit
TCACACAGATTGGGAG182460.5720444994563603No Hit
ATTCCTAACTGCTACA182210.5712607050638134No Hit
TGCGGTGACCTTAGTG181900.5702888000170553No Hit
TCCGCGAGAGAACAGC178040.5581870145961327No Hit
ACTGATTCTCGGCTCA172880.5420094983339666No Hit
AGGTCGAGAAGGGAGG172310.54022244711896No Hit
TGGACCTCTAGGGAGG169720.532102337212175No Hit
TGGCCTGGACCCGAGT168250.5274936261839999No Hit
CCATGATGATTCGGGC162710.5101247424451626No Hit
GCAATCATGGAACGTG154560.48457304524813677No Hit
ACGAGGATGGAGCTGA144430.4528136964621402No Hit
TTCGATGGAAATCCTG144010.4514969218826616No Hit
AAGGTGCGAACAGATA139880.43854863851778836No Hit
TTTCGTAACAGTACGG137370.43067934281661846No Hit
TCCCAGACTGTCTTGA134700.4223084187042186No Hit
TGGTCCTTGGAGAGGG132960.41685320973209283No Hit
TCTCTCTACTCTGGAT131450.41211909160111015No Hit
TCGCCGAGAAGGTAAT117450.368226605618489No Hit
TCCTTCACTTGTCACA116180.36424493010435127No Hit
TTCGATGGACAGGTGA116050.3638373570202269No Hit
GCGGTTCGAGTACGTT114980.3604827170201266No Hit
AGCTGCTCTTGTCACA114750.3597616261789835No Hit
TGATATCCTCGATGTT114650.35944810842196484No Hit
AGCCCAGTGAAAGTAC114180.35797457496397683No Hit
AACTCCCTGCTTCACC111910.35085772187965186No Hit
CTTTCGGTGCTTTGTT111520.34963500262727876No Hit
AACCCTCACGCGGTTC105740.33151367627159667No Hit
CCCTTGCCTCCTACGG105250.3299774392622049No Hit
ATGGCCGTGATACGCT103480.32442817496297355No Hit
TCGGCTTACCTCCTAC103260.3237384358975324No Hit
GGGGGGGGGGGGGGGG102250.32057190655164325No Hit
TGTTTGTTGCAAGTTC99080.31063339365414977No Hit
AAACACGCTGCAGTTT97280.30499007402781275No Hit
ACCCTTGTGGGCCATC94070.29492615402751177No Hit
TGGCCTTTGCGAAAGC92150.28890661309275234No Hit
TACAACGTGAGCAGGT90570.28395303253185655No Hit
CTAACTGGAACCTTGA90370.28332599701781913No Hit
CAATGTGTGGTTGACT89950.2820092224383405No Hit
AAATAGGACTCTATCT89780.28147624225140866No Hit
ACAGCCGACAAACATG85470.26796362692390174No Hit
CTTTCGGTGAGCCATT84790.2658317061761744No Hit
TCAAAGGCTCTTAGTG84330.2643895244938883No Hit
TGAACTGTGTGCTGGC84000.2633549158957265No Hit
GGACATTTGGCCGCTA79160.2481806564560204No Hit
AGTGTTCGATTCGCCT77940.24435573982039194No Hit
CAGCCGATGTAATAGG77070.24162813533432906No Hit
GAGGTTGGAGGAATAT73740.23118799402560564No Hit
CGACGTTCTTGCTACA67330.21109150580070554No Hit
TTGGGAGGAATCTGTG66720.20917904748289132No Hit
GTGCTAATGGGAGTCA65420.20510331664164794No Hit
TGTACTGGAAATGCTC64590.20250111925839256No Hit
AGGGACCCTAATCCTG62380.1955723768282788No Hit
GGAACATCTTTACGAC62090.1946631753329245No Hit
GGGTGAAACCGACTAT60500.18967824299632682No Hit
TCTTGAACTCGTACAT59680.1871073973887733No Hit
GGACCACTGGCTAGAG59580.18679387963175456No Hit
TAACGAGCTTACTTTC59030.18506953196815162No Hit
TACCACCTGAAACATG58360.18296896299612617No Hit
CGGATGAGAACAGGCA58310.1828122041176168No Hit
TACGTGTGAAACGCAG57250.17948891589321836No Hit
AGCTTGTGAACCGTGC56450.17698077383706856No Hit
CATGATGTGCCTAGTA56350.17666725608004988No Hit
ACTTATCTGTCAGGTT53690.16832768374335186No Hit
ACGTGTTGATCTTTGT50820.1593297241169145No Hit
AACTAGCACTGCCACG49680.1557556216869011No Hit
TCGGACCGAATGTCCA49510.15522264149996925No Hit
TGGTAGGGATGTGCTA49320.1546269577616337No Hit
GTCCGAGTGGTCTTGA49160.15412532935040374No Hit
AACGAACCTGACACCC49020.15368640449057755No Hit
CGTCGTACTTGCGCCA48770.15290261009803074No Hit
AACTACGACATTGCCA48360.15161718729425397No Hit
GGCTTGCGAATACGCT48100.15080204112600532No Hit
GTAGATAGAGCTGAGA47530.14901498991099857No Hit
CCTTCAGTGCAACGCC46800.1467263102847619No Hit
CTCGGATCTAGTACGG46700.1464127925277432No Hit
TACCACCTGTACTTTC46530.14587981234081138No Hit
AGTTTGCGAGCAGGAT46460.14566034991089827No Hit
TTTAGGGACTGGGCAA46130.14462574131273645No Hit
GGTAGTTTGTCCAGCG45440.14246246878930727No Hit
TGAGGAGTGGTCCCAG45390.14230570991079794No Hit
GCCATAATGACCGTTA43840.13744618467700775No Hit
CTTTAACTGTTCGCCT43010.13484398729375233No Hit
GATAAGAACATTGGTC42560.1334331573871681No Hit
AGAACGCGACACAGTT41480.1300471656113659No Hit
CGATCGGTGCGAGGAT40970.1284482250505704No Hit
GTCCGTTACGGATGGT40970.1284482250505704No Hit
CTTACATTGGAAGGCT40710.12763307888232175No Hit
GGGATGTGATGCATGT40460.12684928448977495No Hit
CCGTTACTGTTGGTTG39650.12430979065792327No Hit
TGCAATGACTCCGTAA39430.12362005159248209No Hit
CAAAGACCTGCTTTGA39020.12233462878870534No Hit
GAGAAGAGATGCTACA38590.12098650243352482No Hit
GAACCATACTTTGGGT38580.12095515065782296No Hit
CAGGCCATGTCGTAGT38430.12048487402229488No Hit
GAAAGTCCTTATCTGG38350.1202340598166799No Hit
AACTACGACGCTGTTA37270.1168480680408777No Hit
AATTCTGCTTAGCGAG36990.11597021832122527No Hit
CCCACCTCTTGACCTA36770.11528047925578408No Hit
TCCTCATCTTTCTGGA36350.11396370467630546No Hit
CCTGAGGACTGACTCA35230.11045230579769577No Hit
TGTCTTCCTGAAATCA35200.11035825047059016No Hit
ATGGCCGTGGGCCAAA34960.10960580785374521No Hit
GAAGGACACCCAGCTG34270.10744253533031603No Hit
TCGTAGAACCGCACTG34130.10700361047048983No Hit
GTAAAGCCTGAAATCA33800.10596900187232804No Hit
AGGTGATCTGTTAGTC33280.1043387095358307No Hit
TATGGGTACCCTTTGC33130.10386843290030259No Hit
TCCGTAAGATTACGCA33040.10358626691898576No Hit
ACAAGACCTAAGCTAA32580.10214408523669964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)