FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3606339
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC350269297.1259773415644Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA230480.6390968791342133No Hit
ATCTGCTC156220.43318168369640236No Hit
ATCAGATC109290.30304971329650376Illumina PCR Primer Index 9 (100% over 8bp)
ATATGATC79860.2214434083983785No Hit
ATCTGAAC67230.18642174238195577No Hit
AACTGATC65310.18109778365261836No Hit
ATCTCATC54510.15115051580009534No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)