FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628539
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT470461.7898155591376044No Hit
GAAGCAGTGGAACCTT146690.5580666674529082No Hit
GTTCTTCCTAACGTAA140840.535810958102581No Hit
CGCTTAAGATAATAGG111270.42331500502750763No Hit
TGTCCCATGAGACAAT107040.4072224151895787No Hit
CATGGAGGATCGACAT106920.40676588781829No Hit
AAAGCATGATCTTTGT100370.3818471021354448No Hit
TGAGCGCACAGGACAC91880.34954779061676466No Hit
TGTCGAAACTTCGCAA88590.337031331853931No Hit
TAATTGCCTGTCTGGC88040.3349389147355242No Hit
GATACGTACCGCTCGA83620.31812348989305467No Hit
GGAAGGACTAGGTAAT81420.3097538214194273No Hit
GGGGGGGGGGGGGGGG73870.2810306409758425No Hit
CATGTCAGAGGTTTGT71890.27349793934957783No Hit
AATGATGGAAAGCTAA70270.2673348198371795No Hit
TCGACCTTGTTTCGCC65550.24937807656648805No Hit
GTGAGATCTCGATTAC59440.22613322457836843No Hit
ATGGGTACTTGGCTGC54310.20661667945577372No Hit
GCTATGATGACCTGCG54150.20600797629405537No Hit
AAACGACCTAGGGAGG51680.19661112123502827No Hit
AGCCTACCTTAACTCG50420.19181758383649625No Hit
TTGCGATGACATTCAG49670.18896428776594146No Hit
CAGCTCACTGTTTACG49330.18767079354728997No Hit
TGTTCCTCTTTATGGC47930.18234464088225436No Hit
CAATGTGACCAACGCC44840.17058906107156865No Hit
CTGCCTTGACCATAAC44690.1700184018574577No Hit
AGCTTGTGACTCACTC44340.1686868636911988No Hit
CCTGTTGACCCTAGTA42920.1632846231309484No Hit
CATGCAAACGAAGGCT42520.1617628652266525No Hit
CACAGCTCTGGACTGA42450.16149655759340073No Hit
TAACCAGACATCACAC42280.16084981048407498No Hit
CATGATGTGTCATGTG42180.16046937100800104No Hit
ATGCCGCGATGCCACG42070.16005088758431965No Hit
CTATTCAACCGAACGA41730.15875739336566816No Hit
ATGGGTACTTTCGGGC41680.15856717362763117No Hit
AGAACGCGAGGAATAT40000.15217579042958845No Hit
ATTAGCGTGGTCGGTC39810.15145295542504789No Hit
TTGTTTGTGATACGCT39700.15103447200136652No Hit
CACTATACTACGCCAC38150.14513766012221999No Hit
CATTAGTCTATTCTGG38130.1450615722270052No Hit
GCAGATACTCGGCTCA37790.14376807800835367No Hit
GCATCTCTGAGCCTAT36970.14064847430454713No Hit
GGGTCGTACCGCTCGA36070.1372245190198814No Hit
CCCAATGCTGGAATGC35600.1354364534823337No Hit
CCGCTATTGTTCGCAA35240.13406687136846743No Hit
TTGACGTGACCTCAAA35060.13338208031153428No Hit
AGCTCGGACCTTAAGC34710.13205054214527537No Hit
ATAGGAAACAGACTTC34630.13174619056441622No Hit
GGGATCAGATGAGTAA34210.13014834476490553No Hit
AACGTCCCTTACAGAA33980.12927333396993537No Hit
CTGCCATTGCACAGTT33820.12866463080821705No Hit
AACCAACACCGGAATG33740.12836027922735785No Hit
TGGGCCATGCGTTCTC33580.1277515760656395No Hit
TGGAGTTACAAGTCTC33430.12718091685152855No Hit
ATAGCCAACATGGCCC33280.1266102576374176No Hit
TCAGGCTCTTAAAGCA33080.12584937868526966No Hit
CCTACGGGATGGAACG32900.12516458762833652No Hit
CTGATTACTGCTTTGA32880.1250884997331217No Hit
CGATCAAACCGATCGC32850.12497436789029952No Hit
GATACCATGTCTGGAT32840.12493632394269213No Hit
TGTTTGTTGCGCTCGA32800.12478414815226253No Hit
CGCTTACTGGCCACGT32430.12337652209078882No Hit
TTGTAACCTCATCCTC32370.12314825840514444No Hit
GCGTCGATGCTTGAAC31930.12147432471041898No Hit
CATGTTACTAACGTGG31370.11934386364440475No Hit
TCCAAAGTGAAGGCCA31330.11919168785397515No Hit
CGCTGAGTGTTAGCTG31280.11900146811593816No Hit
ATCTTACTGCGCACTG31220.11877320443029378No Hit
CTCGGATCTTTCTGAG30820.1172514465259979No Hit
TTCTAACACTACGTTG30540.1161862159929908No Hit
CGCTTCCCTGCGCTGT30440.11580577651691681No Hit
CTTAGGCCTTATGACC29710.11302856834157682No Hit
TGGGTGCACTTCTGAG29660.11283834860353983No Hit
GTAAGAAACGACAAAT29360.11169703017531792No Hit
ATTGAGAGATGATCAC29270.11135463464685136No Hit
AGTGGTACTCAACGCC29080.11063179964231079No Hit
CCTCCCTTGTTTCGCC28970.11021331621862943No Hit
GCATAGTACGCTCCTT28970.11021331621862943No Hit
TACAACCGATTCCATT28950.11013722832341465No Hit
GTGTACTGAATCACAC28680.10911004173801492No Hit
TATCTCCCTGGAATTA28640.10895786594758534No Hit
TGAGATAGACTCATCC28400.1080448112050078No Hit
GCGACAAGAAAGGCTG28400.1080448112050078No Hit
CGAGTCTCTTACTTTC28200.10728393225285986No Hit
ATCGCAATGTAACTCG28090.10686544882917849No Hit
AACCACTGAAAACATG28000.10652305330071192No Hit
TACGCCTTGGGTTGAG27990.10648500935310451No Hit
TTCACGCTGTGGGCAA27880.10606652592942316No Hit
GCATATCGAGCCGCTA27760.10560999855813438No Hit
CCTTCAGTGTAGCTTG27760.10560999855813438No Hit
AGAACAATGAGTCGGA27530.10473498776316424No Hit
AACAGCTACTCGAGAG27510.10465889986794946No Hit
TGGTCCTTGTTTCTGT27250.10366975723015714No Hit
GGTAGCCTGTGCCACG27250.10366975723015714No Hit
GGTCAGAGAACCAGTT27060.10294692222561658No Hit
GGGCTGACTAATACAG27040.1028708343304018No Hit
CATGTCAGAACAGGTG26940.10249039485432782No Hit
AGGTTACTGGTCCCGA26780.10188169169260947No Hit
ACCTCCATGGAATTGA26440.10058819747395797No Hit
TTCTATGACCAACGCC26370.10032188984070618No Hit
AGCCGGTACGCGCAAA26340.100207757997884No Hit
GAAAGTAACCCTGCGA26330.10016971405027661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)