FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4414001
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA233380.5287266586482423No Hit
GATGAATGACTCCTAC217170.49200260715844873No Hit
CGACCAGACGCGCTGT210490.47686894497758386No Hit
TAGCTTTACCCTATCC200990.45534652121737174No Hit
CCAGGACTGTAACTCG149170.33794736340114107No Hit
ATCTGTACTGGGACTA144390.3271181859723185No Hit
GTTCTCGACCGTAGAG138940.31477111128882845No Hit
CTTCCTTTGGCTCAGC136030.30817845306333186No Hit
CGATCAAACCCGGCTA128100.29021289301928116No Hit
AGACCATTGAATCCAC117260.26565467474973387No Hit
TGCTGCTACTTTCTTG113190.2564340153072009No Hit
GGCAACAGAATAGCAA109140.24725866623047887No Hit
GGGGGGGGGGGGGGGG108350.24546890678094546No Hit
ACAGCCGACTTGCGTA106090.24034883544430552No Hit
AACTTACCTCACCTAA96270.21810144583111782No Hit
CGCGTTGGAGCGGTTC91060.20629809553735942No Hit
GTAACTGTGTGAAAGT86450.19585405621793017No Hit
ATCGAAAGATTTCCGC86420.19578609066921374No Hit
CCCTGAACTAGCTTTG83870.19000901902831469No Hit
AGTGGAGTGAGTTGAA83560.1893067083582446No Hit
CAATTCGGATAACGGG80440.18223829129173283No Hit
GGGTAGAACCAGTCTA74200.1681014571587093No Hit
TTCCTAAACTAATGCC72320.16384228277247787No Hit
AAGAACCCTTAACCCA71670.1623696958836212No Hit
TTTAGTCTGAGCAATA70160.15894876326489277No Hit
GTGAAGCGATCCCAGG70000.158586280338405No Hit
CGCGATCGATATCTGG68170.15444038186670098No Hit
TGGAACACTCCTAGTA66940.15165379436932613No Hit
TGCAGATACTGCCACG65530.14845941357965256No Hit
CTTTAACTGTCCGTAA63790.14451741175409794No Hit
AGTCCTGCTCGAACGA63150.14306748004814682No Hit
CACATCCTGAGCCTAT63050.14284092821909194No Hit
TGATAGAGAGACTGTT62450.14148161724476274No Hit
GCAACTTGAAGGTGCA62200.14091523767212558No Hit
TCTCAGAGATGCATGT61370.13903485749097022No Hit
CATAGGTACAAGGTGC60240.13647482182265025No Hit
TGCAGTATGACACCGC59860.13561392487224175No Hit
TCCGGGAACGTTCTCC59580.13497957975088815No Hit
AGGTGTGGATCATGTG58740.13307654438682728No Hit
TCAAAGCACTCTGCAA58630.13282733737486693No Hit
TGGCAATTGAGTGGTG58040.13149068158344324No Hit
CTATTGGGACTTGTGA57580.13044854316979085No Hit
CTTAGATCTTGTCACA57540.1303579224381689No Hit
ATGTACGTGTACTTTC55940.12673309317329107No Hit
ATTTCTGACGGGTAGT55480.1256909547596387No Hit
TACGTTTACTGCACCG55310.12530581665024546No Hit
TGAACTGTGAACGTAA54980.12455819561436438No Hit
AGAGTACCTTAATCGC54790.12412774713916014No Hit
CGAGTCTCTGCTACGC54460.1233801261032791No Hit
TCTATGGGATGCTCAA53930.12217940140928832No Hit
TTCTTAGACGTCCCGA53470.12113726299563593No Hit
TCCATTTGAAATCTGC52740.11948343464353542No Hit
GGGTGTGCTCGCAAAG52190.11823739958373367No Hit
TACTGTTTGCTTAAGC51410.11647029531710573No Hit
GGTCTAAACTCCTACC51410.11647029531710573No Hit
GAGTGTTACACCTGCG51390.11642498495129476No Hit
CTACAACGAATCTGTG51090.11574532946413017No Hit
GGTCGCATGGTACTAC50890.11529222580602043No Hit
CATTCTATGCCTAGCG49800.11282281086932242No Hit
GCATGATACTTGCGTA49790.11280015568641694No Hit
CACCAGGTGCGCCACA49620.11241501757702367No Hit
CTCAATTTGGAATCTC48840.11064791331039572No Hit
GTAATCTCTACACAAA48820.11060260294458474No Hit
CTAGCTCACGAATAGT48420.10969639562836528No Hit
GGGATGTGACTGGAAG48190.10917532642153908No Hit
ACCAAGTTGAAGCTAA47840.10838239501984707No Hit
CTTTCGTCTCGAACGA47730.10813318800788672No Hit
TTGCGGTCTTTTGGGT47640.10792929136173735No Hit
TGCGCAGACATCAGGG47430.10745353252072214No Hit
AGACTACTGGCCCTAG47060.10661529075321913No Hit
TCGGACCGATAGGCGA46830.10609422154639295No Hit
CACATTACTGATGGAC46600.10557315233956675No Hit
CATAGTCGAAGATTCC46500.1053466005105119No Hit
AGCCAATTGTTATGGC46470.10527863496179543No Hit
ACCTCAGCTAATCCGT46320.10493880721821314No Hit
CGAGTCTCTTGGAACG46030.10428180691395403No Hit
TGCTAGTGAAGGCGTA45690.10351153069516748No Hit
TGTAAGCACGCGCTGT45640.10339825478064006No Hit
ATGTCCAGACCGCTAG45520.10312639258577422No Hit
TGAGGGATGTACGTTG44580.1009968053926585No Hit
TAGTACCGAACGAGCA44510.1008382191123201No Hit
CTAACAGTGCATGATC44400.10058901210035975No Hit
TCCGGGTGAACACCTA44200.10013590844225001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)