FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043380
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA296554997.44261314722446No Hit
GGCAAGGC97370.3199403295020668No Hit
GGCAAGAA93610.30758564490796414No Hit
GGCAACGA76440.2511681091418094No Hit
GGCAAGCA64710.21262543619265423Clontech Universal Primer Mix Long (100% over 8bp)
GCCAAGGA47770.15696363911177705RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA46640.15325066209280472No Hit
GGCAGGGA44680.14681045416609165No Hit
GGAAAGGA43730.14368892481385828No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)