FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386139
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC235000598.48567078447651Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGATA68630.28761945553046153No Hit
TGTAGCTC49380.20694519472671125No Hit
TATAGATC38240.16025889522781364No Hit
AGTAGATC23910.10020371822429457No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)