FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998621
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT539311.7985267227835728No Hit
GAAGCAGTGGAACCTT166720.5559889028990326No Hit
GTTCTTCCTAACGTAA159650.5324113984394827No Hit
CGCTTAAGATAATAGG126510.42189393057675517No Hit
CATGGAGGATCGACAT123240.41098891790593073No Hit
TGTCCCATGAGACAAT121670.40575317787743104No Hit
AAAGCATGATCTTTGT114680.38244246271869636No Hit
TGTCGAAACTTCGCAA104050.3469928343728667No Hit
TGAGCGCACAGGACAC103690.34579228251919797No Hit
TAATTGCCTGTCTGGC101040.336954886929692No Hit
GATACGTACCGCTCGA95880.3197469770271068No Hit
GGAAGGACTAGGTAAT92950.30997581888474735No Hit
GGGGGGGGGGGGGGGG83910.2798286278926213No Hit
CATGTCAGAGGTTTGT80270.2676897147055263No Hit
AATGATGGAAAGCTAA78440.2615869094493769No Hit
TCGACCTTGTTTCGCC75210.2508152914289602No Hit
GTGAGATCTCGATTAC67280.22436980198564607No Hit
GCTATGATGACCTGCG63930.21319800001400646No Hit
ATGGGTACTTGGCTGC61650.2055945049407711No Hit
AAACGACCTAGGGAGG59460.19829114783095295No Hit
AGCCTACCTTAACTCG57430.19152136932276537No Hit
CAGCTCACTGTTTACG56430.18818650306257442No Hit
TTGCGATGACATTCAG56260.187619575798342No Hit
TGTTCCTCTTTATGGC55630.1855186100544217No Hit
CTGCCTTGACCATAAC51260.1709452444973873No Hit
CCTGTTGACCCTAGTA50130.16717684562337154No Hit
AGCTTGTGACTCACTC49770.1659762937697028No Hit
CATGCAAACGAAGGCT49430.1648424392412379No Hit
CACAGCTCTGGACTGA49390.16470904459083027No Hit
CAATGTGACCAACGCC49220.16414211732659778No Hit
CATGATGTGTCATGTG48800.1627414734973176No Hit
TAACCAGACATCACAC47750.15923986392411713No Hit
ATGGGTACTTTCGGGC47180.1573389901558083No Hit
ATGCCGCGATGCCACG46010.1534371966313849No Hit
CTATTCAACCGAACGA45960.15327045331837535No Hit
TTGTTTGTGATACGCT45870.15297031535495817No Hit
ATTAGCGTGGTCGGTC45770.15263682872893908No Hit
CATTAGTCTATTCTGG44310.1477679239890603No Hit
CACTATACTACGCCAC43510.14510003098090757No Hit
AGAACGCGAGGAATAT43470.14496663633049992No Hit
GCATCTCTGAGCCTAT43050.1435659925012197No Hit
GCAGATACTCGGCTCA42930.1431658085499968No Hit
CCGCTATTGTTCGCAA41500.13839694979792377No Hit
GGGTCGTACCGCTCGA41150.13722974660685697No Hit
AGCTCGGACCTTAAGC40360.1345952022613061No Hit
GGGATCAGATGAGTAA40140.1338615316840641No Hit
CCCAATGCTGGAATGC39780.13266097983039538No Hit
ATAGGAAACAGACTTC39590.1320273552409591No Hit
TTGACGTGACCTCAAA39220.13079345472468845No Hit
AACGTCCCTTACAGAA39070.1302932247856598No Hit
TTGTAACCTCATCCTC38730.1291593702571949No Hit
CTGCCATTGCACAGTT38520.1284590483425548No Hit
TCAGGCTCTTAAAGCA38430.12815891037913762No Hit
CTGATTACTGCTTTGA37890.1263580825986345No Hit
CGCTTACTGGCCACGT37870.12629138527343067No Hit
AACCAACACCGGAATG37760.1259245499848097No Hit
GATACCATGTCTGGAT37700.12572445800919824No Hit
TGGAGTTACAAGTCTC37670.12562441202139252No Hit
ATAGCCAACATGGCCC37430.12482404411894668No Hit
TGGGCCATGCGTTCTC37030.1234900976148703No Hit
CGCTGAGTGTTAGCTG36660.12225619709859965No Hit
CCTACGGGATGGAACG36470.12162257250916336No Hit
TTCTAACACTACGTTG36440.12152252652135764No Hit
CGATCAAACCGATCGC35860.1195883040904469No Hit
CATGTTACTAACGTGG35510.11842110089938009No Hit
TGTTTGTTGCGCTCGA35400.11805426561075907No Hit
CTCGGATCTTTCTGAG35190.11735394369611898No Hit
ATCTTACTGCGCACTG35160.11725389770831326No Hit
TCCAAAGTGAAGGCCA35110.1170871543953037No Hit
TGGGTGCACTTCTGAG35060.11692041108229416No Hit
CGAGTCTCTTACTTTC35050.11688706241969225No Hit
CGCTTCCCTGCGCTGT34690.11568651056602351No Hit
GCGTCGATGCTTGAAC34550.11521962928959678No Hit
GTAAGAAACGACAAAT34100.11371893947251085No Hit
AGTGGTACTCAACGCC33720.11245169029363832No Hit
TACGCCTTGGGTTGAG33710.1124183416310364No Hit
CTTAGGCCTTATGACC33330.11115109245216385No Hit
CCTCCCTTGTTTCGCC33250.11088430315134856No Hit
ATTGAGAGATGATCAC33160.11058416518793138No Hit
GCATAGTACGCTCCTT32950.10988384327329129No Hit
TACAACCGATTCCATT32630.10881668607003019No Hit
TATCTCCCTGGAATTA32270.10761613421636144No Hit
ATCGCAATGTAACTCG31960.10658232567570226No Hit
TGAGATAGACTCATCC31930.10648227968789653No Hit
TTCACGCTGTGGGCAA31890.1063488850374889No Hit
GCGACAAGAAAGGCTG31810.10608209573667363No Hit
CCTTCAGTGTAGCTTG31770.105948701086266No Hit
AACAGCTACTCGAGAG31760.10591535242366408No Hit
AACCACTGAAAACATG31660.10558186579764499No Hit
GGTAGCCTGTGCCACG31570.1052817278342278No Hit
GAAAGTAACCCTGCGA31530.10514833318382016No Hit
TGGTCCTTGTTTCTGT31410.10474814923259726No Hit
GTGTACTGAATCACAC31390.10468145190739343No Hit
AGAACAATGAGTCGGA31370.10461475458218962No Hit
GCATATCGAGCCGCTA30980.10331415674071515No Hit
GGTCAGAGAACCAGTT30960.10324745941551133No Hit
TTCTATGACCAACGCC30690.10234704552525978No Hit
CATGTCAGAACAGGTG30590.10201355889924069No Hit
AGGTTACTGGTCCCGA30530.10181346692362922No Hit
AGCCGGTACGCGCAAA30510.10174676959842542No Hit
ACCTCCATGGAATTGA30470.10161337494801778No Hit
ATTCCTAACATTGCCA30420.10144663163500824No Hit
GGGCTGACTAATACAG30230.10081300704557195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)