FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4636861
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGTCC447433296.49484856242186No Hit
CCCAGTCC461130.9944874344950172No Hit
ACCAGTCA299810.6465796580919722No Hit
ACCAGACC106460.2295949781544023No Hit
ACAAGTCC102850.22180953882378618No Hit
ACCACTCC97260.20975396933399557No Hit
ACCAGTAC57510.12402787144147732No Hit
ACCCGTCC56930.12277702523323429TruSeq Adapter, Index 16 (100% over 8bp)
AACAGTCC51290.11061362417376756No Hit
ACCAGTCG47360.10213806279722425No Hit
AGCAGTCC46750.10082251764717554No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)