FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087486
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT344171.1147257023999462No Hit
TGCTGCTACCGTTCTC313931.0167819384444172No Hit
AAACGACCTCTTCAAG309631.0028547497867197No Hit
ATGGGTTACAATCGAA301300.9758748703637847No Hit
TATGCTTTGGACTGTT295440.9568950272163177No Hit
GCCTCTTGATCTGTGA276000.8939311789591919No Hit
AAAGCGGACTCGGGCT273020.8842793133312993No Hit
TCCAAAGTGCTCACGG262130.8490078983354095No Hit
TAGTGCATGAACCCGC241630.7826108361301071No Hit
TAACTCACTGGTGTCC231990.7513880224881991No Hit
GCAGGTCTGGCTGTTA230560.7467564225392439No Hit
TGTGGCTCTTACGATC228650.7405701596703596No Hit
GAAGTATGAACAGGTG218990.7092825684067879No Hit
TTCCCATCTCTTTGTT208670.6758573156283137No Hit
TGAGAATGAGCAGTTT196270.6356951901968139No Hit
GAGCCTGACCGAGTTT195560.6333955846277521No Hit
TCACCTGACTCAATGC195180.6321648098161416No Hit
GGAACTACTACCAAGG192660.6240028294865143No Hit
CTAGAGACTAATCTGC181060.5864318089215628No Hit
ATGTCCAGAGTCGGTC179940.582804262108395No Hit
TCACACAGATTGGGAG179460.581249599188466No Hit
ATTCCTAACTGCTACA176560.571856844047228No Hit
TCGTATACTGCTAATC176460.5715329559389095No Hit
TTCGTCCGACCTGTGG175650.5689094622615293No Hit
TCCGCGAGAGAACAGC171690.5560834931721148No Hit
AGGTCGAGAAGGGAGG171100.5541725533330354No Hit
TGGACCTCTAGGGAGG167530.5426097478660632No Hit
ACTGATTCTCGGCTCA165210.5350955437530729No Hit
TGGCCTGGACCCGAGT161640.5235327382861007No Hit
CCATGATGATTCGGGC160830.5209092446087205No Hit
GCAATCATGGAACGTG156670.507435499302669No Hit
TTCGATGGAAATCCTG145630.4716782521443012No Hit
ACGAGGATGGAGCTGA139180.4507874691577549No Hit
TTTCGTAACAGTACGG137940.4467712566146049No Hit
AAGGTGCGAACAGATA135470.43877122033913674No Hit
TCCCAGACTGTCTTGA134510.43566189449927867No Hit
TGGTCCTTGGAGAGGG131410.42562136314140375No Hit
TCTCTCTACTCTGGAT130780.42358086805899686No Hit
TGCGGTGACCTTAGTG123710.4006819788008755No Hit
TTCGATGGACAGGTGA113230.3667385050490917No Hit
TCCTTCACTTGTCACA113120.3663822281299413No Hit
TGATATCCTCGATGTT113030.36609072883245464No Hit
AGCTGCTCTTGTCACA112450.36421217780420706No Hit
AGCCCAGTGAAAGTAC111160.3600340212068978No Hit
TCGCCGAGAAGGTAAT109090.35332953736470385No Hit
AACTCCCTGCTTCACC107750.3489894367132353No Hit
AACCCTCACGCGGTTC106350.34445500319677563No Hit
CTTTCGGTGCTTTGTT105760.34254406335769616No Hit
ATGGCCGTGATACGCT102220.3310784243232196No Hit
CCCTTGCCTCCTACGG101390.3283901530241756No Hit
TCGGCTTACCTCCTAC97530.3158880720430797No Hit
AAACACGCTGCAGTTT94310.30545887495522245No Hit
TGTTTGTTGCAAGTTC94010.3044872106302668No Hit
GAAGTAATGAGTCTGT93930.304228100143612No Hit
GGGGGGGGGGGGGGGG92840.3006977197629398No Hit
ACCCTTGTGGGCCATC91650.29684345127394907No Hit
TGGCCTTTGCGAAAGC91160.2952563995431882No Hit
CTAACTGGAACCTTGA90950.29457623451571924No Hit
TACAACGTGAGCAGGT89500.2898798569451003No Hit
CAATGTGTGGTTGACT88560.286835308726906No Hit
AAATAGGACTCTATCT87450.2832401507245701No Hit
TCAAAGGCTCTTAGTG85860.2780903298023052No Hit
ACAGCCGACAAACATG83370.2700255159051734No Hit
GGACATTTGGCCGCTA82740.2679850208227665No Hit
CTTTCGGTGAGCCATT81360.26351536492797056No Hit
TGAACTGTGTGCTGGC79890.25875420973568786No Hit
AGTGTTCGATTCGCCT76820.24881084481030843No Hit
CAGCCGATGTAATAGG76130.24657601686291047No Hit
GGCTTGCGAATACGCT70880.2295718911761867No Hit
GATAAGAACATTGGTC66100.21409003959856013No Hit
TTGGGAGGAATCTGTG65560.21234104381363997No Hit
GTAAAGCCTGAAATCA65400.2118228228403303No Hit
GTGCTAATGGGAGTCA63400.2053450606739593No Hit
TGTACTGGAAATGCTC62150.20129645931997747No Hit
CGACGTTCTTGCTACA61310.19857579921010166No Hit
GGAACATCTTTACGAC61080.19783085656096905No Hit
AGGGACCCTAATCCTG60510.1959846943435533No Hit
GGACCACTGGCTAGAG59670.1932640342336775No Hit
TCTTGAACTCGTACAT58470.18937737693385492No Hit
GGGTGAAACCGACTAT57660.18675388325647468No Hit
TACGTGTGAAACGCAG57630.1866567168239791No Hit
TACCACCTGAAACATG56590.18328828049746623No Hit
TAACGAGCTTACTTTC55480.17969312249513034No Hit
CGGATGAGAACAGGCA55070.1783651812510243No Hit
AGCTTGTGAACCGTGC54660.17703724000691826No Hit
CATGATGTGCCTAGTA53530.17337730438291868No Hit
ACTTATCTGTCAGGTT53140.17211414076047632No Hit
AACTAGCACTGCCACG52840.17114247643552066No Hit
TCGGACCGAATGTCCA49080.15896428356274328No Hit
AACTACGACATTGCCA47920.15520718150624813No Hit
CGTCGTACTTGCGCCA47230.15297235355885014No Hit
TGGTAGGGATGTGCTA46790.15154724588224852No Hit
CCTTCAGTGCAACGCC46750.15141769063892113No Hit
ACGTGTTGATCTTTGT46690.15122335777393No Hit
GTAGATAGAGCTGAGA46540.15073752561145215No Hit
AACGAACCTGACACCC45900.14866464171821347No Hit
TTTAGGGACTGGGCAA45030.1458468151758421No Hit
AGTTTGCGAGCAGGAT44670.14468081798589533No Hit
GGTAGTTTGTCCAGCG44320.1435472096067804No Hit
CTCGGATCTAGTACGG44300.1434824319851167No Hit
GCCATAATGACCGTTA43610.14124760403771872No Hit
TACCACCTGTACTTTC43570.1411180487943913No Hit
GTCCGAGTGGTCTTGA42760.13849455511701106No Hit
TGAGGAGTGGTCCCAG42080.13629211598044494No Hit
AGAACGCGACACAGTT41700.13506134116883445No Hit
CTTTAACTGTTCGCCT41690.13502895235800258No Hit
CAGGCCATGTCGTAGT40310.13055929646320663No Hit
CTTACATTGGAAGGCT39690.12855119019163164No Hit
GGGATGTGATGCATGT38260.1239195902426764No Hit
GTCCGTTACGGATGGT38240.1238548126210127No Hit
CAAAGACCTGCTTTGA38190.12369286856685344No Hit
TGCAATGACTCCGTAA38050.12323942521520745No Hit
GAACCATACTTTGGGT37440.12126370775446431No Hit
GAAAGTCCTTATCTGG37300.12081026440281833No Hit
CGATCGGTGCGAGGAT36890.1194823231587123No Hit
CCGTTACTGTTGGTTG36740.11899649099623448No Hit
GAGAAGAGATGCTACA36330.11766854975212844No Hit
AACTACGACGCTGTTA36170.11715032877881876No Hit
TCCTCATCTTTCTGGA35470.11488311202058892No Hit
GCTGGACACATTGGTC35310.11436489104727923No Hit
AATTCTGCTTAGCGAG34920.1131017274248369No Hit
TGTCTTCCTGAAATCA34860.11290739455984578No Hit
GGCTTAAACTGCATCA34470.11164423093740344No Hit
TCGTAGAACCGCACTG34060.1103162896932974No Hit
ATAGCCAACATCTGTG33710.10918268131418249No Hit
CCCACCTCTTGACCTA33190.10749846315092602No Hit
CCTGAGGACTGACTCA33120.10727174147510306No Hit
ATGGCCGTGGGCCAAA32830.10633246596097926No Hit
TATGGGTACCCTTTGC32730.1060085778526607No Hit
ACAAGACCTAAGCTAA32630.10568468974434216No Hit
TCCGTAAGATTACGCA32580.10552274569018288No Hit
GAGGTTGGAGGAATAT31730.10276969676947523No Hit
AGGTGATCTGTTAGTC31270.1012798114712099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)