FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16950839
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC16600.012.14701222
CTTATAC23150.09.918041
TATACAC36700.09.6117443
CCCACGA20750.09.58043819
CACGAGA21550.09.29067821
TCTCCGA21850.09.28908111
ATCTCCG21100.09.22122310
TAGACCG5950.09.1749525
GACTCTA25500.08.40341926
TTATACA32000.08.3990062
CTTAGAC18600.08.27841953
CCACGAG25300.08.13450520
TCTATAC15200.08.1041373
CTAGTAC12450.08.095263
ACTCTAT25800.07.871488627
GTATAGG14250.07.85973931
TAGGACT24450.07.84325934
TCCGAGC28950.07.832877613
TACACAT38250.07.8313835
TAGGACA23100.07.75626854
Produced by FastQC (version 0.11.5)