FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17774318
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG27600.010.1411765
TATACCG3850.09.0875475
ATACCGT4250.08.8938636
TACACCG8700.08.84730155
CTAGGAC26050.08.7544693
TACACTG30800.08.633175
GTATAGG13400.08.3556141
TAGGACT23000.08.3348484
CTAACAC30200.08.2000453
TAGGACC18150.08.172114
CTACACT27100.08.1581714
CTAGACA24150.08.0538344
GTCCTAC14600.07.95643231
TCTAGAC18150.07.9398173
GTCTAGG23150.07.91978551
TAGACTG22450.07.9168935
TATACTG18250.07.89844855
TAGGACA18550.07.84502654
AGGACTG38800.07.7548635
TAATACT19600.07.7103254
Produced by FastQC (version 0.11.5)