FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612875
Sequences flagged as poor quality0
Sequence length8
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA151618594.00511508951406No Hit
TTAGTCAA724404.491358598775479No Hit
TCAGACAA38160.23659614043245758No Hit
TCAGGCAA34920.21650778888630554No Hit
ACAGTCAA31810.1972254514454003TruSeq Adapter, Index 13 (100% over 8bp)
TCAGTAAA31730.19672944276524837No Hit
TCAGTCAT17970.11141594977912114No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)