FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034278
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC17950.011.15339422
CTTATAC24350.010.6946731
CCCACGA22950.09.08905119
ATCTCCG22950.08.90627310
CACGAGA23050.08.86775721
TATACAC38900.08.853453
TAGACAG32600.08.8467035
TACACCG9400.08.6383725
TCTCCGA24050.08.55712911
GTATAGG17050.08.5400911
TACACTG33500.08.4836565
TAGGACT25800.08.3565694
TTATACA35350.08.3167192
CTAGGAC27750.08.1729543
CTATACT21700.08.1290134
CTAGTAC11400.07.9824373
GTGTAGG29250.07.8978991
CCACGAG27600.07.862082520
ACTGTCC33500.07.85678538
TAGACTG25100.07.8088335
Produced by FastQC (version 0.11.5)