FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015198
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT290869596.46779415481173No Hit
CTTTCCTA540621.7929834126979387No Hit
CTTTCCAT76170.25262022593541117No Hit
CTTTACTT58400.19368545614583188No Hit
CTTTCATT54500.18075098219088764No Hit
CATTCCTT44930.14901177302452442TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT42270.14018979848089577No Hit
ATTTCCTT37090.12301016384330317No Hit
CTTACCTT31580.10473607371721526No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)