FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012363
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC292375997.05865461765399Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC212960.706953312067636No Hit
TGTAGATA146520.4863955638812454No Hit
AGTAGATC61530.20425825174456066No Hit
TGTAGAAC54650.18141903880773996Illumina DpnII expression Adapter 1 (100% over 8bp)
TGAAGATC52890.17557644945180909No Hit
TGTACATC47710.15838064668833074No Hit
TATAGATC38990.12943327215212774No Hit
TTTAGATC33500.11120837694527518No Hit
TCTAGATC32160.10676004186746417ABI Dynabead EcoP Oligo (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)