FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004947783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820803
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC51120.6228047412107413No Hit
CTCCTAGTGAACGTAA42650.5196131105758629No Hit
CGACCAGACGCGCTGT42130.513277851079979No Hit
TAGCTTTACCCTATCC38140.46466691764040824No Hit
CCAGGACTGTAACTCG29880.36403375718656No Hit
ATCTGTACTGGGACTA26560.3235855619436089No Hit
GTTCTCGACCGTAGAG26290.32029610028228456No Hit
CTTCCTTTGGCTCAGC25700.3131080173927239No Hit
CGATCAAACCCGGCTA24460.29800086013330845No Hit
AGACCATTGAATCCAC24320.2962952133459551No Hit
TGCTGCTACTTTCTTG21840.26608089882712416No Hit
ACAGCCGACTTGCGTA19870.24208001189079476No Hit
GGGGGGGGGGGGGGGG18560.22612003123770258No Hit
GGCAACAGAATAGCAA17970.218931948348142No Hit
CGCGTTGGAGCGGTTC17810.21698263773402388No Hit
AACTTACCTCACCTAA17730.21600798242696484No Hit
ATCGAAAGATTTCCGC16960.20662692509652134No Hit
GTAACTGTGTGAAAGT16430.20016983368725505No Hit
CCCTGAACTAGCTTTG16070.19578388480548928No Hit
AGTGGAGTGAGTTGAA15330.1867683232151929No Hit
CAATTCGGATAACGGG15050.18335702964048622No Hit
AAGAACCCTTAACCCA14330.17458513187695465No Hit
GGGTAGAACCAGTCTA14200.17300131700298366No Hit
TTCCTAAACTAATGCC13670.16654422559371737No Hit
TCCGGGAACGTTCTCC13130.1599653022710687No Hit
GTGAAGCGATCCCAGG12830.1563103448695972No Hit
CGCGATCGATATCTGG12670.1543610342554791No Hit
TGGAACACTCCTAGTA12630.15387370660194954No Hit
TTTAGTCTGAGCAATA12600.15350821086180239No Hit
CACATCCTGAGCCTAT12360.15058424494062522No Hit
AGTCCTGCTCGAACGA12260.1493659258068014No Hit
CTTTAACTGTCCGTAA12230.14900043006665423No Hit
TGATAGAGAGACTGTT12140.14790394284621278No Hit
TGCAGATACTGCCACG12090.1472947832793009No Hit
TGCAGTATGACACCGC11960.1457109684053299No Hit
ATTTCTGACGGGTAGT11800.14376165779121178No Hit
TACGTTTACTGCACCG11490.1399848684763579No Hit
GCAACTTGAAGGTGCA11410.13901021316929885No Hit
TCTCAGAGATGCATGT11310.137791894035475No Hit
GAGTGTTACACCTGCG11240.13693907064179833No Hit
TGAACTGTGAACGTAA11150.1358425834213569No Hit
AGGTGTGGATCATGTG11140.13572075150797452No Hit
TGGCAATTGAGTGGTG10940.13328411324032685No Hit
TCAAAGCACTCTGCAA10860.1323094579332678No Hit
CATAGGTACAAGGTGC10750.13096930688606157No Hit
CTTAGATCTTGTCACA10700.13036014731914966No Hit
CTATTGGGACTTGTGA10690.13023831540576727No Hit
AGAGTACCTTAATCGC10640.12962915583885537No Hit
ATGTACGTGTACTTTC10540.12841083670503153No Hit
CGAGTCTCTGCTACGC10500.12792350905150202No Hit
TTCTTAGACGTCCCGA10390.1265833580042958No Hit
TCTATGGGATGCTCAA10390.1265833580042958No Hit
GGTCTAAACTCCTACC10330.12585236652400147No Hit
GGTCGCATGGTACTAC10310.12560870269723673No Hit
CACCAGGTGCGCCACA10300.12548687078385434No Hit
TGAGGGATGTACGTTG10270.1251213750437072No Hit
TACTGTTTGCTTAAGC10210.12439038356341289No Hit
GTAATCTCTACACAAA10010.12195374529576525No Hit
CTAGCTCACGAATAGT9880.12036993042179427No Hit
TTGCGGTCTTTTGGGT9880.12036993042179427No Hit
TCCGGGTGAACACCTA9810.11951710702811759No Hit
TCGGACCGATAGGCGA9750.11878611554782328No Hit
GGGTGTGCTCGCAAAG9610.11708046876046992No Hit
GGGATGTGACTGGAAG9530.11610581345341087No Hit
AGACTACTGGCCCTAG9520.11598398154002848No Hit
CATTCTATGCCTAGCG9410.11464383049282227No Hit
TCCATTTGAAATCTGC9390.1144001666660575No Hit
ATGTCCAGACCGCTAG9380.11427833475267511No Hit
TGTAAGCACGCGCTGT9340.1137910070991456No Hit
CGAGTCTCTTGGAACG9290.11318184753223368No Hit
CTTTCGTCTCGAACGA9280.11306001561885129No Hit
CTCAATTTGGAATCTC9170.11171986457164508No Hit
CTACAACGAATCTGTG9080.11062337735120364No Hit
ACCTCAGCTAATCCGT9040.1101360496976741No Hit
GGGCCTTGACTATCTT9040.1101360496976741No Hit
TGCGCAGACATCAGGG8940.10891773056385028No Hit
AGGCGATGACCTGCGA8810.1073339156898793No Hit
CATAGTCGAAGATTCC8760.10672475612296739No Hit
GGCTGACTGAAACTGA8750.10660292420958502No Hit
TAGTACCGAACGAGCA8730.10635926038282024No Hit
AGCCAATTGTTATGGC8710.10611559655605547No Hit
ACCAAGTTGAAGCTAA8660.10550643698914355No Hit
GTCTAATCTAAGTTCC8610.10489727742223165No Hit
TGCTAGTGAAGGCGTA8460.1030697987214959No Hit
AGCTTGTGATTGCGTA8460.1030697987214959No Hit
CACATTACTGATGGAC8420.10258247106796636No Hit
ATAGCCAACCGTTCTC8300.10112048810737777No Hit
GTGTGATACCGTGCAC8300.10112048810737777No Hit
TCATCATTGGACTCGC8250.10051132854046586No Hit
AAAGCCTTGAACCAAA8230.10026766471370109No Hit
CTAACAGTGCATGATC8220.1001458328003187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)