FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295761
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA417726997.24165287593979No Hit
GCCAAAAA146710.34152272437875386No Hit
CCCAACAA142350.3313731839364434RNA PCR Primer, Index 36 (100% over 8bp)
GCCCACAA110810.25795196706706913No Hit
GCCACCAA95470.22224234541912366No Hit
GACAACAA83030.19328356489106355No Hit
GCAAACAA77860.18124844468768164No Hit
GCCAACCA73220.170447098895865No Hit
GCCAACAC64190.14942637637429085No Hit
TCCAACAA54630.1271718794411514No Hit
ACCAACAA48510.11292527680194497No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)