FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3349537
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT326672197.5275388807468No Hit
CTAGCTGA192270.5740196331612399No Hit
CTAGCTAT58840.17566606966873333RNA PCR Primer, Index 38 (100% over 8bp)
CTAGCAGT58240.1738747773199699No Hit
CAAGCTGT54190.16178355396581678No Hit
CTAGATGT47340.1413329663174343No Hit
CTAGCTGG46810.13975065807602663No Hit
CTAGCGGT42330.12637567520525972No Hit
CTCGCTGT36020.10753725067076435No Hit
ATAGCTGT33920.10126772745009235No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)