FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445697
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC153560.627878269466741No Hit
CTCCTAGTGAACGTAA126620.5177256217757147No Hit
TAGCTTTACCCTATCC111800.4571293991038138No Hit
CCAGGACTGTAACTCG86840.35507260302482274No Hit
ATCTGTACTGGGACTA80810.3304170549336242No Hit
CTTCCTTTGGCTCAGC78180.31966347425703184No Hit
GTTCTCGACCGTAGAG77760.3179461723999334No Hit
CGATCAAACCCGGCTA72170.29508970244474275No Hit
CGACCAGACGCGCTGT65610.26826708296244384No Hit
GGGGGGGGGGGGGGGG62910.2572272853096684No Hit
TGCTGCTACTTTCTTG62170.2542015629900188No Hit
ACAGCCGACTTGCGTA58630.23972716162304653No Hit
AACTTACCTCACCTAA54870.2243532211880703No Hit
GGCAACAGAATAGCAA52490.214621843997846No Hit
CGCGTTGGAGCGGTTC49680.20313227681106857No Hit
ATCGAAAGATTTCCGC49230.201292310535606No Hit
AGTGGAGTGAGTTGAA47820.19552708287248993No Hit
GTAACTGTGTGAAAGT47690.1949955370595785No Hit
CCCTGAACTAGCTTTG47400.19380978101539154No Hit
CAATTCGGATAACGGG45700.18685879730808844No Hit
GGGTAGAACCAGTCTA41080.167968476880006No Hit
TTCCTAAACTAATGCC40090.1639205510739883No Hit
CGCGATCGATATCTGG39640.16208058479852575No Hit
AAGAACCCTTAACCCA39170.16015884224415372No Hit
GTGAAGCGATCCCAGG38560.15766466573741555No Hit
TTTAGTCTGAGCAATA37820.154638943417766No Hit
TCCGGGAACGTTCTCC36870.1507545701695672No Hit
TGGAACACTCCTAGTA36550.14944614970701603No Hit
TGCAGATACTGCCACG36490.1492008208702877No Hit
CTTTAACTGTCCGTAA35320.14441690855408498No Hit
AGTCCTGCTCGAACGA35150.1437218101833547No Hit
TGATAGAGAGACTGTT34440.14081875228206928No Hit
CACATCCTGAGCCTAT34370.14053253530588622No Hit
AGGTGTGGATCATGTG34310.14028720646915788No Hit
TGCAGTATGACACCGC34190.13979654879570116No Hit
CATAGGTACAAGGTGC34190.13979654879570116No Hit
GCAACTTGAAGGTGCA33970.13889700972769725No Hit
TCTCAGAGATGCATGT33570.13726148414950828No Hit
CTATTGGGACTTGTGA32790.13407220927203983No Hit
TCAAAGCACTCTGCAA32760.13394954485367566No Hit
TACGTTTACTGCACCG32010.13088293439457135No Hit
ATTTCTGACGGGTAGT31650.1294109613742013No Hit
CTTAGATCTTGTCACA31540.12896119184019933No Hit
TGAGGGATGTACGTTG31280.12789810021437653No Hit
AGAGTACCTTAATCGC31030.12687589672800842No Hit
CGAGTCTCTGCTACGC31010.12679412044909896No Hit
TGGCAATTGAGTGGTG30800.12593546952054976No Hit
TGAACTGTGAACGTAA30480.1246270490579986No Hit
ATGTACGTGTACTTTC30470.12458616091854387No Hit
TCTATGGGATGCTCAA30320.12397283882672301No Hit
GGTCTAAACTCCTACC30060.1229097472009002No Hit
NNNNNNNNNNNNNNNN30040.12282797092199076No Hit
GGTCGCATGGTACTAC29870.12213287255126044No Hit
TTCTTAGACGTCCCGA29790.12180576743562264No Hit
GAGTGTTACACCTGCG29570.12090622836761873No Hit
TCCATTTGAAATCTGC29380.12012935371797896No Hit
GGGTGTGCTCGCAAAG29210.11943425534724865No Hit
CTACAACGAATCTGTG29210.11943425534724865No Hit
TACTGTTTGCTTAAGC29060.1188209332554278No Hit
GCATGATACTTGCGTA28390.11608142791196129No Hit
GGGATGTGACTGGAAG28370.11599965163305184No Hit
AGACTACTGGCCCTAG28060.11473211930995542No Hit
TCCGGGTGAACACCTA28040.11465034303104595No Hit
CACCAGGTGCGCCACA27820.11375080396304202No Hit
CTTTCGTCTCGAACGA27340.11178817326921528No Hit
TCGGACCGATAGGCGA27330.11174728512976055No Hit
TTGCGGTCTTTTGGGT27250.11142018001412275No Hit
CTAGCTCACGAATAGT27200.11121573931684914No Hit
CATTCTATGCCTAGCG27150.11101129861957551No Hit
CGAGTCTCTTGGAACG26850.1097846544359338No Hit
CTCAATTTGGAATCTC26830.10970287815702436No Hit
GTAATCTCTACACAAA26360.10778113560265233No Hit
AGCCAATTGTTATGGC26220.1072087016502862No Hit
CACATTACTGATGGAC26130.10684070839519368No Hit
ACCAAGTTGAAGCTAA26100.10671804397682952No Hit
GGCTGACTGAAACTGA25900.10590028118773503No Hit
AGGCGATGACCTGCGA25740.10524607095645944No Hit
CATAGTCGAAGATTCC25560.10451008444627441No Hit
TGCTAGTGAAGGCGTA25110.10267011817081184No Hit
ATGTCCAGACCGCTAG25090.10258834189190238No Hit
TGCGCAGACATCAGGG25060.10246567747353823No Hit
TGTAAGCACGCGCTGT25060.10246567747353823No Hit
ACGAGGATGAACTAGA25060.10246567747353823No Hit
TTGTGCGCTATGTGGG24620.10066659933753036No Hit
TAGTACCGAACGAGCA24550.10038038236134729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)