FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100583
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT336781.0861828243269087No Hit
TGCTGCTACCGTTCTC320201.0327090098862053No Hit
AAACGACCTCTTCAAG306840.989620339142671No Hit
ATGGGTTACAATCGAA296220.9553687161414482No Hit
TATGCTTTGGACTGTT296040.9547881801583767No Hit
GCAGGTCTGGCTGTTA281240.9070552215502699No Hit
AAAGCGGACTCGGGCT274950.8867687141418242No Hit
TCCAAAGTGCTCACGG263040.848356583261922No Hit
TAGTGCATGAACCCGC251430.8109120123538057No Hit
GAAGTATGAACAGGTG248000.7998495766763863No Hit
TAACTCACTGGTGTCC235190.7585347658811262No Hit
TGTGGCTCTTACGATC232350.7493751981482192No Hit
GCCTCTTGATCTGTGA217560.7016744915391718No Hit
TTCCCATCTCTTTGTT209030.6741635363413913No Hit
GGAACTACTACCAAGG198300.6395571413505138No Hit
TCACCTGACTCAATGC197800.6379445413975371No Hit
TGAGAATGAGCAGTTT192710.6215282738762355No Hit
GAAGTAATGAGTCTGT190860.6155616540502222No Hit
ATGTCCAGAGTCGGTC184850.5961782026154436No Hit
CTAGAGACTAATCTGC182250.5877926828599653No Hit
TCGTATACTGCTAATC180970.5836644269803454No Hit
TTCGTCCGACCTGTGG179250.5781170831421059No Hit
TCACACAGATTGGGAG178400.5753756632220457No Hit
TGCGGTGACCTTAGTG177940.5738920712653072No Hit
ATTCCTAACTGCTACA174190.5617975716179828No Hit
TCCGCGAGAGAACAGC174110.5615395556255066No Hit
CCATGATGATTCGGGC172210.5554116758041955No Hit
AGGTCGAGAAGGGAGG169640.5471229120458959No Hit
ACTGATTCTCGGCTCA167100.5389309042847747No Hit
TGGACCTCTAGGGAGG165370.5333513084474759No Hit
GCAATCATGGAACGTG161530.5209665408086156No Hit
TGGCCTGGACCCGAGT159060.5130002970409113No Hit
TTCGATGGAAATCCTG144480.4659768824121141No Hit
ACGAGGATGGAGCTGA141380.4559787627036593No Hit
AAGGTGCGAACAGATA139070.44852855092090743No Hit
TTTCGTAACAGTACGG137190.44246517509771544No Hit
TGGTCCTTGGAGAGGG132060.42591989958017573No Hit
TCCCAGACTGTCTTGA131800.4250813476046279No Hit
GCGGTTCGAGTACGTT130860.42204965969303193No Hit
TCTCTCTACTCTGGAT128660.4149542198999349No Hit
TCCTTCACTTGTCACA115710.3731878811178414No Hit
TCGCCGAGAAGGTAAT113640.3665117173125183No Hit
TGATATCCTCGATGTT112340.3623189574347792No Hit
TTCGATGGACAGGTGA112210.3618996814470053No Hit
AGCTGCTCTTGTCACA111650.36009356949967153No Hit
CTTTCGGTGCTTTGTT109850.3542882096689558No Hit
AACTCCCTGCTTCACC108320.3493536538128475No Hit
AGCCCAGTGAAAGTAC105520.3403230940761786No Hit
AACCCTCACGCGGTTC100800.3251001505200796No Hit
ATGGCCGTGATACGCT100510.3241648425473532No Hit
TCGGCTTACCTCCTAC99200.31993983067055454No Hit
CCCTTGCCTCCTACGG98950.31913353069406625No Hit
TGTTTGTTGCAAGTTC95860.3091676629846709No Hit
AAACACGCTGCAGTTT93740.3023302391840502No Hit
CTAACTGGAACCTTGA91120.2938802154304529No Hit
TGGCCTTTGCGAAAGC88750.2862364916533439No Hit
GGGGGGGGGGGGGGGG88120.2842046157125934No Hit
TACAACGTGAGCAGGT86900.28026987182733054No Hit
CAATGTGTGGTTGACT86750.27978609184143755No Hit
TCAAAGGCTCTTAGTG86540.2791087998611874No Hit
AAATAGGACTCTATCT86240.2781412398894015No Hit
ACAGCCGACAAACATG84490.2724971400539834No Hit
GGACATTTGGCCGCTA84330.2719811080690309No Hit
ACCCTTGTGGGCCATC84010.2709490440991259No Hit
CTTTCGGTGAGCCATT81520.2629182963333025No Hit
TGAACTGTGTGCTGGC80040.2581450004724918No Hit
AGTGTTCGATTCGCCT75170.24243827693049982No Hit
GAGGTTGGAGGAATAT74730.24101918897188046No Hit
GATAAGAACATTGGTC74440.240083880999154No Hit
CAGCCGATGTAATAGG74010.23869704503959419No Hit
GGCTTGCGAATACGCT71010.22902144532173466No Hit
GTGCTAATGGGAGTCA68290.220248901577542No Hit
CGACGTTCTTGCTACA66790.21541110171861225No Hit
GTAAAGCCTGAAATCA66630.21489506973365974No Hit
TGTACTGGAAATGCTC63700.20544523400921696No Hit
TTGGGAGGAATCTGTG63060.20338110606940696No Hit
GGAACATCTTTACGAC61940.19976888217473943No Hit
AGGGACCCTAATCCTG59470.19180263840703507No Hit
TACGTGTGAAACGCAG58770.18954499847286785No Hit
TCTTGAACTCGTACAT58100.18738411453587922No Hit
TAACGAGCTTACTTTC57860.18661006655845044No Hit
GGGTGAAACCGACTAT57720.18615853857161702No Hit
TACCACCTGAAACATG55920.18035317874090132No Hit
CGGATGAGAACAGGCA55710.17967588676065113No Hit
AGCTTGTGAACCGTGC54000.17416079492147124No Hit
CATGATGTGCCTAGTA53800.17351575494028057No Hit
ACTTATCTGTCAGGTT52500.16932299506254148No Hit
AACTAGCACTGCCACG52110.16806516709921973No Hit
TCGGACCGAATGTCCA50220.16196953927696825No Hit
ACGTGTTGATCTTTGT49960.16113098730142042No Hit
AACTACGACATTGCCA49030.15813155138888396No Hit
TGGTAGGGATGTGCTA48610.15677696742838362No Hit
AACGAACCTGACACCC48230.15555139146412142No Hit
GGTAGTTTGTCCAGCG48180.15539013146882377No Hit
CGTCGTACTTGCGCCA47640.15364852351960903No Hit
GTAGATAGAGCTGAGA47580.15345501152525187No Hit
CTCGGATCTAGTACGG46670.15052007961083447No Hit
TTTAGGGACTGGGCAA46090.14864946366538165No Hit
CCTTCAGTGCAACGCC45200.14577903574908332No Hit
AGTTTGCGAGCAGGAT45050.14529525576319036No Hit
TACCACCTGTACTTTC44200.14255383584313014No Hit
CTTTAACTGTTCGCCT42890.13832882396633148No Hit
TGAGGAGTGGTCCCAG42870.13826431996821242No Hit
GCCATAATGACCGTTA41870.13503912006225927No Hit
GAGAAGAGATGCTACA41750.1346520960735449No Hit
GAAAGTCCTTATCTGG41540.1339748040932947No Hit
CAGGCCATGTCGTAGT41540.1339748040932947No Hit
CTTACATTGGAAGGCT40980.13216869214596094No Hit
GGGATGTGATGCATGT40500.13062059619110342No Hit
GGACCACTGGCTAGAG40290.12994330421085323No Hit
AGAACGCGACACAGTT39820.12842746025505525No Hit
CGATCGGTGCGAGGAT39170.1263310803161857No Hit
TGCAATGACTCCGTAA39030.12587955232935225No Hit
GAAGGACACCCAGCTG38800.12513775635098304No Hit
CAAAGACCTGCTTTGA38210.12323488840647065No Hit
GCTGGACACATTGGTC38000.12255759642622048No Hit
TCCTCATCTTTCTGGA37180.11991293250333888No Hit
AATTCTGCTTAGCGAG36610.11807456855694558No Hit
CCGTTACTGTTGGTTG36250.11691349659080244No Hit
AACTACGACGCTGTTA36010.11613944861337368No Hit
CCCACCTCTTGACCTA35400.11417207667074225No Hit
GGCTTAAACTGCATCA34890.11252722471870613No Hit
TCGTAGAACCGCACTG34650.11175317674127737No Hit
TGTCTTCCTGAAATCA34600.11159191674597971No Hit
GCGTTTAGAAGTCTAC34380.11088237276667No Hit
ACAAGACCTAAGCTAA33360.10759266886259777No Hit
ATGGCCGTGGGCCAAA33030.10652835289363322No Hit
ATAGCCAACATCTGTG33020.1064961008945737No Hit
CCTGAGGACTGACTCA32740.1055930449209068No Hit
TCCGTAAGATTACGCA32600.10514151693407336No Hit
AGGTGATCTGTTAGTC31830.10265811300648943No Hit
ACTGTCCACGTTGGTT31470.10149704104034628No Hit
TATGGGTACCCTTTGC31390.10123902504787002No Hit
GGCTTCTACATCAGCT31380.10120677304881051No Hit
GCGTTAATGGTACGTT31010.10001344908360783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)