FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4105130
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT398573297.09149283944723No Hit
CTAGCTGA230020.5603233027943086No Hit
CTAGCAGT87460.2130505002277638No Hit
CAAGCTGT77110.187838143980824No Hit
CTAGATGT69830.17010423543225184No Hit
CTAGCTGG67420.16423353219021078No Hit
CTAGCTCT66950.16308862325919032No Hit
CTCGCTGT65690.16001929293347592No Hit
CTAGCTAT64100.1561460903795982RNA PCR Primer, Index 38 (100% over 8bp)
CTAGCGGT63970.15582941344123086No Hit
CTACCTGT61280.1492766367934755No Hit
ATAGCTGT55080.1341735828098014No Hit
CTAGCTTT41360.10075198592980003No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)