FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4283303
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG417849097.55298656200601No Hit
TAATGGTG108360.2529823362951442No Hit
TATTGGGG100220.23397831066352298No Hit
TATTGGTA98580.23014948977459684No Hit
AATTGGTG81730.19081068978776425No Hit
TTTTGGTG70470.16452256587964942RNA PCR Primer, Index 28 (100% over 8bp)
TATTGGTC69230.1616276037441199No Hit
TATTGGTT64360.15025787342151606No Hit
TATTGGAG63940.1492773217304496No Hit
TATTGCTG58390.1363200315270715No Hit
TATAGGTG57540.13433558167610368RNA PCR Primer, Index 39 (100% over 8bp)
TATTCGTG55780.13022660316115858No Hit
TAGTGGTG50460.11780628174098352No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)