FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004947865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14438578
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1591801.1024631372978697No Hit
TGCTGCTACCGTTCTC1473041.0202112701126107No Hit
AAACGACCTCTTCAAG1443810.999966894246788No Hit
ATGGGTTACAATCGAA1391260.9635713433829841No Hit
TATGCTTTGGACTGTT1375320.95253147505246No Hit
GCCTCTTGATCTGTGA1288000.892054605377344No Hit
AAAGCGGACTCGGGCT1276450.8840552026660796No Hit
TCCAAAGTGCTCACGG1226480.8494465313689479No Hit
TAGTGCATGAACCCGC1148060.7951337036098707No Hit
TAACTCACTGGTGTCC1084670.7512304882101271No Hit
TGTGGCTCTTACGATC1077160.746029144975357No Hit
GCAGGTCTGGCTGTTA1061010.7348438329591737No Hit
GAAGTATGAACAGGTG1015260.7031578871548154No Hit
TTCCCATCTCTTTGTT984560.6818954054893771No Hit
TCACCTGACTCAATGC927440.642334722990034No Hit
GAGCCTGACCGAGTTT920150.6372857493307167No Hit
GGAACTACTACCAAGG904090.6261627703226731No Hit
TGAGAATGAGCAGTTT899240.6228037137729214No Hit
ATGTCCAGAGTCGGTC859750.5954533749791704No Hit
CTAGAGACTAATCTGC854210.5916164320336809No Hit
TTCGTCCGACCTGTGG838890.5810059688703416No Hit
TCGTATACTGCTAATC838080.5804449717970841No Hit
TCACACAGATTGGGAG831840.5761232165660635No Hit
ATTCCTAACTGCTACA810570.5613918489757094No Hit
TCCGCGAGAGAACAGC793180.5493477266251566No Hit
ACTGATTCTCGGCTCA785060.5437239041129951No Hit
AGGTCGAGAAGGGAGG784530.54335683195395No Hit
TGGACCTCTAGGGAGG774420.5363547573729213No Hit
TGGCCTGGACCCGAGT756430.5238950816347704No Hit
CCATGATGATTCGGGC751670.5205983580931585No Hit
GCAATCATGGAACGTG722170.5001669832029165No Hit
TTCGATGGAAATCCTG665300.46077944794840603No Hit
ACGAGGATGGAGCTGA645630.44715622272498023No Hit
AAGGTGCGAACAGATA635100.4398632607726329No Hit
TCCCAGACTGTCTTGA631360.4372729779899378No Hit
TTTCGTAACAGTACGG631030.4370444236267588No Hit
TGGTCCTTGGAGAGGG610910.4231095333626344No Hit
TCTCTCTACTCTGGAT601780.41678619598134947No Hit
TGCGGTGACCTTAGTG575540.39861266116372407No Hit
TCCTTCACTTGTCACA538290.3728137216836727No Hit
TGATATCCTCGATGTT524760.36344299279333464No Hit
AGCCCAGTGAAAGTAC520990.36083193234125965No Hit
TTCGATGGACAGGTGA520910.36077652522291326No Hit
AGCTGCTCTTGTCACA513970.35596995770636136No Hit
TCGCCGAGAAGGTAAT512580.3550072590250924No Hit
AACTCCCTGCTTCACC497140.3443136851842335No Hit
CTTTCGGTGCTTTGTT495410.3431155062499922No Hit
AACCCTCACGCGGTTC492970.3414255891404264No Hit
CCCTTGCCTCCTACGG475980.3296585023816057No Hit
ATGGCCGTGATACGCT475330.32920831954504104No Hit
TCGGCTTACCTCCTAC456330.3160491289377666No Hit
TGTTTGTTGCAAGTTC440310.3049538534888962No Hit
AAACACGCTGCAGTTT438250.30352712019147593No Hit
GGGGGGGGGGGGGGGG432090.29926077207880164No Hit
ACCCTTGTGGGCCATC429820.29768859509572204No Hit
CTAACTGGAACCTTGA425450.2946619812560489No Hit
GAAGTAATGAGTCTGT422640.29271580622413096No Hit
TGGCCTTTGCGAAAGC420830.2914622201715432No Hit
TACAACGTGAGCAGGT416770.28865030891546245No Hit
CAATGTGTGGTTGACT408250.2827494508115688No Hit
AAATAGGACTCTATCT405410.28078249811027095No Hit
TCAAAGGCTCTTAGTG402910.2790510256619454No Hit
ACAGCCGACAAACATG392200.27163339769331857No Hit
GGACATTTGGCCGCTA384420.2662450554341293No Hit
CTTTCGGTGAGCCATT382430.26486680336526214No Hit
TGAACTGTGTGCTGGC377030.26112682287687883No Hit
CAGCCGATGTAATAGG355890.24648549185383767No Hit
AGTGTTCGATTCGCCT353900.24510723978497054No Hit
GGCTTGCGAATACGCT332540.23031353918647668No Hit
GATAAGAACATTGGTC311710.21588691074702787No Hit
GTGCTAATGGGAGTCA302370.20941812968008344No Hit
GTAAAGCCTGAAATCA301720.2089679468435188No Hit
TTGGGAGGAATCTGTG297510.20605214724053852No Hit
TGTACTGGAAATGCTC293640.2033718278905305No Hit
CGACGTTCTTGCTACA283590.19641130864826162No Hit
GGAACATCTTTACGAC283370.196258939072809No Hit
AGGGACCCTAATCCTG280530.1942919863715111No Hit
GGACCACTGGCTAGAG279570.19362710095135408No Hit
TCTTGAACTCGTACAT272210.18852964606348355No Hit
GGGTGAAACCGACTAT267170.18503899760765916No Hit
TACCACCTGAAACATG264100.18291274944111532No Hit
TAACGAGCTTACTTTC263510.18250412194331048No Hit
TACGTGTGAAACGCAG261820.18133364656824238No Hit
AGCTTGTGAACCGTGC260420.18036402199718005No Hit
CGGATGAGAACAGGCA258830.17926280552004498No Hit
CATGATGTGCCTAGTA250740.17365976067726338No Hit
ACTTATCTGTCAGGTT248320.1719836953472842No Hit
AACTAGCACTGCCACG243710.1687908601525718No Hit
TCGGACCGAATGTCCA232120.16076375388213437No Hit
AACTACGACATTGCCA226860.15712073585085734No Hit
ACGTGTTGATCTTTGT226270.1567121083530525No Hit
TGGTAGGGATGTGCTA226190.15665670123470607No Hit
CGTCGTACTTGCGCCA220200.15250809324851797No Hit
GTAGATAGAGCTGAGA218350.15122680363675703No Hit
AACGAACCTGACACCC217710.15078354668998567No Hit
TTTAGGGACTGGGCAA215550.14928755449463235No Hit
GTCCGAGTGGTCTTGA214510.1485672619561289No Hit
CTCGGATCTAGTACGG211330.14636482900185876No Hit
CCTTCAGTGCAACGCC209800.14530516786348352No Hit
GGTAGTTTGTCCAGCG207970.14403773003130918No Hit
AGTTTGCGAGCAGGAT207270.143552917745778No Hit
TACCACCTGTACTTTC204580.14168985339137968No Hit
GCCATAATGACCGTTA203870.14119811521605521No Hit
TGAGGAGTGGTCCCAG202950.1405609333550714No Hit
CTTTAACTGTTCGCCT195690.13553273736513388No Hit
CAGGCCATGTCGTAGT189680.13137027759935915No Hit
AGAACGCGACACAGTT189150.13100320544031413No Hit
CTTACATTGGAAGGCT188990.1308923912036213No Hit
GTCCGTTACGGATGGT183040.1267714867766064No Hit
TGCAATGACTCCGTAA182550.12643211817673458No Hit
CAAAGACCTGCTTTGA180410.12494997776096788No Hit
GGGATGTGATGCATGT178990.12396650141031894No Hit
GAAAGTCCTTATCTGG178140.12337780077788824No Hit
GAGAAGAGATGCTACA177780.12312846874532936No Hit
GAACCATACTTTGGGT174940.12116151604403147No Hit
CGATCGGTGCGAGGAT173740.1203304092688352No Hit
CCGTTACTGTTGGTTG173520.12017803969338253No Hit
AACTACGACGCTGTTA169670.11751157212296114No Hit
AATTCTGCTTAGCGAG169030.11706831517618978No Hit
TCCTCATCTTTCTGGA168130.11644498509479258No Hit
GCTGGACACATTGGTC163840.11347377837346585No Hit
GGCTTAAACTGCATCA161120.11158993634968763No Hit
TCGTAGAACCGCACTG160910.11144449266402828No Hit
TGTCTTCCTGAAATCA160190.1109458285989105No Hit
CCCACCTCTTGACCTA159700.11060645999903869No Hit
ATGGCCGTGGGCCAAA159590.11053027521131235No Hit
ATAGCCAACATCTGTG157770.10926976326893134No Hit
CCTGAGGACTGACTCA156680.10851484128146138No Hit
ACAAGACCTAAGCTAA155930.10799539954696369No Hit
TCCGTAAGATTACGCA154670.1071227374330076No Hit
TATGGGTACCCTTTGC151080.10463634299721204No Hit
ACTGTCCACGTTGGTT147680.10228154046748926No Hit
AGGTGATCTGTTAGTC147470.1021360967818299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)