FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870973
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT401971.0384210894780201No Hit
TGCTGCTACCGTTCTC378930.9789011703259102No Hit
AAACGACCTCTTCAAG366470.9467128807150038No Hit
ATGGGTTACAATCGAA356570.9211379154543314No Hit
TATGCTTTGGACTGTT348450.9001612772809318No Hit
GCCTCTTGATCTGTGA341500.882207135001975No Hit
AAAGCGGACTCGGGCT322760.8337955340943994No Hit
TCCAAAGTGCTCACGG311300.8041905743078032No Hit
TAGTGCATGAACCCGC304450.7864947650112775No Hit
GCAGGTCTGGCTGTTA289220.7471506517870313No Hit
TAACTCACTGGTGTCC283800.7331490041392693No Hit
GAAGTATGAACAGGTG278850.7203615215089333No Hit
TGTGGCTCTTACGATC276090.7132315311938368No Hit
TTCCCATCTCTTTGTT249140.6436107924286737No Hit
GAGCCTGACCGAGTTT245150.6333033064296755No Hit
GGAACTACTACCAAGG238840.6170024952382773No Hit
TCACCTGACTCAATGC234860.6067208425375222No Hit
TGAGAATGAGCAGTTT228700.5908075308197707No Hit
CTAGAGACTAATCTGC228540.590394198047881No Hit
ATGTCCAGAGTCGGTC228100.5892575329251845No Hit
GAAGTAATGAGTCTGT221040.5710192243655536No Hit
TCGTATACTGCTAATC217030.5606600717700692No Hit
TTCGTCCGACCTGTGG212390.5486734213852693No Hit
TGCGGTGACCTTAGTG210490.5437650947190796No Hit
ATTCCTAACTGCTACA209900.5422409301227366No Hit
TCACACAGATTGGGAG205390.530590112615097No Hit
ACTGATTCTCGGCTCA201670.5209801256686626No Hit
AGGTCGAGAAGGGAGG201030.519326794581104No Hit
TCCGCGAGAGAACAGC200480.5179059631777333No Hit
TGGCCTGGACCCGAGT198510.5128168034238421No Hit
TGGACCTCTAGGGAGG191280.49413932879407835No Hit
CCATGATGATTCGGGC185360.4788460162341613No Hit
GCAATCATGGAACGTG176680.4564227133591477No Hit
ACGAGGATGGAGCTGA175030.4521602191490357No Hit
TTCGATGGAAATCCTG171500.44304106486922024No Hit
AAGGTGCGAACAGATA162570.41997192953812906No Hit
TCCCAGACTGTCTTGA160550.41475360329302224No Hit
TTTCGTAACAGTACGG159410.4118086072933084No Hit
TGGTCCTTGGAGAGGG152860.39488779694407583No Hit
TCTCTCTACTCTGGAT152660.3943711309792137No Hit
GCGGTTCGAGTACGTT149630.38654364161155347No Hit
TCCTTCACTTGTCACA140140.3620278415788485No Hit
TCGCCGAGAAGGTAAT138310.35730034800036065No Hit
AGCCCAGTGAAAGTAC136910.3536836862463262No Hit
TTCGATGGACAGGTGA135300.34952452522918653No Hit
TGATATCCTCGATGTT132910.34335036694908494No Hit
AGCTGCTCTTGTCACA130050.3359620436515574No Hit
AACTCCCTGCTTCACC127480.32932288600307985No Hit
AACCCTCACGCGGTTC126300.3262745568103937No Hit
CCCTTGCCTCCTACGG124420.32141789674069027No Hit
CTTTCGGTGCTTTGTT124200.320849564179342No Hit
ATGGCCGTGATACGCT123010.3177754016884127No Hit
GGGGGGGGGGGGGGGG118260.30550458502293865No Hit
TCGGCTTACCTCCTAC116750.30160375698823005No Hit
TGTTTGTTGCAAGTTC113010.2919421034453095No Hit
CTAACTGGAACCTTGA112060.2894879401122147No Hit
ACCCTTGTGGGCCATC111250.28739544295452335No Hit
AAACACGCTGCAGTTT111020.286801277094932No Hit
AAATAGGACTCTATCT109640.2832362819373837No Hit
TGGCCTTTGCGAAAGC108860.28122128467442165No Hit
TACAACGTGAGCAGGT107010.27644212449944755No Hit
CAATGTGTGGTTGACT104040.2687696349212459No Hit
CTTTCGGTGAGCCATT99580.2572479839048219No Hit
TCAAAGGCTCTTAGTG98780.2551813200453736No Hit
TGAACTGTGTGCTGGC95010.24544216660772367No Hit
CAGCCGATGTAATAGG92700.23947467471356684No Hit
AGTGTTCGATTCGCCT91300.2358580129595324No Hit
GAGGTTGGAGGAATAT89640.23156968545117723No Hit
ACAGCCGACAAACATG82860.21405470924235329No Hit
GGCTTGCGAATACGCT82400.21286637752317056No Hit
GATAAGAACATTGGTC81590.21077388036547917No Hit
GTGCTAATGGGAGTCA81080.2094563821550809No Hit
CGACGTTCTTGCTACA80330.20751888478684816No Hit
GTAAAGCCTGAAATCA78290.2022488919452551No Hit
TGTACTGGAAATGCTC77750.20085389384012753No Hit
TTGGGAGGAATCTGTG77730.20080222724364133No Hit
AGGGACCCTAATCCTG71440.18455308264872938No Hit
GGAACATCTTTACGAC71030.18349391742076218No Hit
TAACGAGCTTACTTTC70270.18153058675428632No Hit
TCTTGAACTCGTACAT70230.18142725356131392No Hit
GGGTGAAACCGACTAT69850.180445588228076No Hit
CGGATGAGAACAGGCA69170.178688923947545No Hit
GGACCACTGGCTAGAG67760.17504642889526742No Hit
TACGTGTGAAACGCAG67290.17383226387784156No Hit
GGACATTTGGCCGCTA67030.1731605981235209No Hit
TACCACCTGAAACATG66410.17155893363244848No Hit
AGCTTGTGAACCGTGC66040.17060310159745365No Hit
CATGATGTGCCTAGTA64620.166934773246933No Hit
AACTAGCACTGCCACG63900.16507477577342958No Hit
ACGTGTTGATCTTTGT63460.16393811065073305No Hit
ACTTATCTGTCAGGTT63060.1629047787210089No Hit
TGGTAGGGATGTGCTA59940.15484478966916068No Hit
TCGGACCGAATGTCCA58820.15195146026593312No Hit
AACTACGACATTGCCA58280.15055646216080557No Hit
CTCGGATCTAGTACGG57680.14900646426621939No Hit
GTCCGAGTGGTCTTGA57350.14815396542419695No Hit
GTAGATAGAGCTGAGA56970.14717230009095905No Hit
GGTAGTTTGTCCAGCG56840.1468364672137987No Hit
AACGAACCTGACACCC56540.1460614682665056No Hit
CGTCGTACTTGCGCCA56300.14544146910867112No Hit
TTTAGGGACTGGGCAA54920.14187647395112288No Hit
TGAGGAGTGGTCCCAG54140.13986147668816085No Hit
AGTTTGCGAGCAGGAT53920.13929314412681257No Hit
CCTTCAGTGCAACGCC53670.138647311670735No Hit
TACCACCTGTACTTTC53500.13820814560060224No Hit
GCCATAATGACCGTTA52640.13598648195169535No Hit
CTTTAACTGTTCGCCT50320.1299931567592954No Hit
GTCCGTTACGGATGGT48460.1251881632860782No Hit
AGAACGCGACACAGTT48300.12477483051418854No Hit
GAGAAGAGATGCTACA47480.1226565000582541No Hit
CTTACATTGGAAGGCT47450.12257900016352477No Hit
CCGTTACTGTTGGTTG46940.1212615019531265No Hit
GGGATGTGATGCATGT46880.12110650216366789No Hit
CGATCGGTGCGAGGAT46720.12069316939177825No Hit
GAAAGTCCTTATCTGG46500.12012483683042996No Hit
CAGGCCATGTCGTAGT46450.11999567033921445No Hit
GAACCATACTTTGGGT45660.1179548397780093No Hit
TGCAATGACTCCGTAA45590.11777400669030758No Hit
AACTACGACGCTGTTA45040.11635317528693691No Hit
CAAAGACCTGCTTTGA44510.11498401048005243No Hit
AATTCTGCTTAGCGAG43960.11356317907668174No Hit
GCTGGACACATTGGTC43880.11335651269073693No Hit
TCCTCATCTTTCTGGA43650.11276234683114555No Hit
GAAGGACACCCAGCTG42470.10971401763845937No Hit
TGTCTTCCTGAAATCA42220.10906818518238179No Hit
GGCTTAAACTGCATCA42130.10883568549819388No Hit
CCCACCTCTTGACCTA41960.1083965194280611No Hit
ATGGCCGTGGGCCAAA41750.10785402016495595No Hit
ATAGCCAACATCTGTG40850.10552902332307665No Hit
ACAAGACCTAAGCTAA40830.10547735672659045No Hit
CCTGAGGACTGACTCA40320.1041598585161922No Hit
GCGTTTAGAAGTCTAC40150.10372069244605944No Hit
AGGTGATCTGTTAGTC40040.10343652616538529No Hit
TCCGTAAGATTACGCA39820.10286819360403703No Hit
TCGTAGAACCGCACTG39650.10242902753390427No Hit
GCGTTAATGGTACGTT39420.10183486167431288No Hit
TATGGGTACCCTTTGC39050.10087902963931807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)