FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17401297
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTA183330.10535421583804931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTA22050.024.19770628
GACCCGT25900.020.49296226
ACCCGTT26950.019.95481927
ACGAGAC28300.019.64273522
TCCGAGC30900.018.52797313
ATCTCCG30750.018.43621410
TCTCCGA30950.018.27185211
AGACCCG30800.017.77793725
CACGAGA31450.017.62985221
CCCACGA31950.017.57059719
CCACGAG32400.017.37031720
CTCCGAG34000.016.9621712
CGAGCCC33850.016.789715
GCCCACG35400.016.21370918
CCGAGCC37100.015.62075414
CTTATAC48150.013.0600271
AGCGTCA9900.012.4426553
TATACAC70900.012.2408353
ACATCTC56800.011.4594888
TACACAT67900.011.0705725
Produced by FastQC (version 0.11.5)