FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004947886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17401297
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1867761.0733452799524081No Hit
TGCTGCTACCGTTCTC1778331.0219525590535006No Hit
AAACGACCTCTTCAAG1735120.9971210766645727No Hit
ATGGGTTACAATCGAA1681510.9663130282759956No Hit
TATGCTTTGGACTGTT1661470.9547966453305177No Hit
GCAGGTCTGGCTGTTA1558620.8956918556128316No Hit
AAAGCGGACTCGGGCT1533910.8814917646655879No Hit
TCCAAAGTGCTCACGG1466250.8426096054794077No Hit
TAGTGCATGAACCCGC1383960.7953200270071823No Hit
GAAGTATGAACAGGTG1378200.7920099289150687No Hit
TAACTCACTGGTGTCC1309480.7525186197327705No Hit
TGTGGCTCTTACGATC1301520.7479442480638081No Hit
TTCCCATCTCTTTGTT1173810.6745531669277296No Hit
GCCTCTTGATCTGTGA1169320.6719728994913425No Hit
TCACCTGACTCAATGC1117820.6423774043969251No Hit
GGAACTACTACCAAGG1101150.6327976587032564No Hit
TGAGAATGAGCAGTTT1080160.6207353394405026No Hit
GAAGTAATGAGTCTGT1045670.6009149777743579No Hit
ATGTCCAGAGTCGGTC1031970.5930420014094352No Hit
CTAGAGACTAATCTGC1027900.5907030952922647No Hit
TCACACAGATTGGGAG1009720.5802555981890316No Hit
TCGTATACTGCTAATC1007090.5787442165948895No Hit
TTCGTCCGACCTGTGG1006760.5785545755583621No Hit
TGCGGTGACCTTAGTG989100.5684059067551114No Hit
ATTCCTAACTGCTACA973030.5591709629460379No Hit
CCATGATGATTCGGGC966880.5556367436289376No Hit
TCCGCGAGAGAACAGC963930.5539414676963448No Hit
AGGTCGAGAAGGGAGG956040.5494073229139185No Hit
ACTGATTCTCGGCTCA936550.5382070083626525No Hit
TGGACCTCTAGGGAGG922950.5303914989784957No Hit
GCAATCATGGAACGTG903950.5194727726329825No Hit
TGGCCTGGACCCGAGT879440.5053876156472704No Hit
TTCGATGGAAATCCTG808150.4644194050592895No Hit
ACGAGGATGGAGCTGA794770.4567303230328176No Hit
AAGGTGCGAACAGATA768190.4414555995452523No Hit
TTTCGTAACAGTACGG756220.434576801947579No Hit
TCCCAGACTGTCTTGA744300.4277267378402886No Hit
TGGTCCTTGGAGAGGG738350.42430745248472No Hit
GCGGTTCGAGTACGTT723400.415716138860224No Hit
TCTCTCTACTCTGGAT718060.41264740208732714No Hit
TCCTTCACTTGTCACA645420.3709033872590072No Hit
TCGCCGAGAAGGTAAT638670.3670243660573117No Hit
TTCGATGGACAGGTGA633200.3638809222094192No Hit
TGATATCCTCGATGTT629940.3620074986364522No Hit
AGCTGCTCTTGTCACA623280.358180197717446No Hit
CTTTCGGTGCTTTGTT606180.3483533440064841No Hit
AACTCCCTGCTTCACC605480.3479510751411231No Hit
AGCCCAGTGAAAGTAC583080.3350784714495707No Hit
AACCCTCACGCGGTTC565310.3248665889674775No Hit
CCCTTGCCTCCTACGG558320.32084964701194396No Hit
TCGGCTTACCTCCTAC552040.31724072061984804No Hit
ATGGCCGTGATACGCT549370.3157063522333996No Hit
AAACACGCTGCAGTTT529770.30444282400329126No Hit
TGTTTGTTGCAAGTTC528980.303988834855241No Hit
CTAACTGGAACCTTGA512810.2946964240654016No Hit
GGGGGGGGGGGGGGGG510880.29358731133662047No Hit
TACAACGTGAGCAGGT498680.2865763396831857No Hit
TGGCCTTTGCGAAAGC493760.2837489642295054No Hit
CAATGTGTGGTTGACT490290.2817548599969301No Hit
TCAAAGGCTCTTAGTG485690.2791113788817006No Hit
AAATAGGACTCTATCT484640.2785079755836591No Hit
ACAGCCGACAAACATG475610.273318707220502No Hit
ACCCTTGTGGGCCATC467670.2687558289476928No Hit
CTTTCGGTGAGCCATT462730.26591696009785937No Hit
GGACATTTGGCCGCTA461940.2654629709498091No Hit
TGAACTGTGTGCTGGC450390.2588255346713524No Hit
AGTGTTCGATTCGCCT428780.24640692012785023No Hit
GAGGTTGGAGGAATAT426000.24480933806255936No Hit
CAGCCGATGTAATAGG425310.24441281589527492No Hit
GATAAGAACATTGGTC407920.23441930794009203No Hit
GGCTTGCGAATACGCT401180.23054603343647317No Hit
GTGCTAATGGGAGTCA382270.21967902737364925No Hit
CGACGTTCTTGCTACA374470.2151966028739122No Hit
GTAAAGCCTGAAATCA370410.21286344345481834No Hit
TTGGGAGGAATCTGTG362990.20859939348199158No Hit
TGTACTGGAAATGCTC356900.20509965435335079No Hit
GGAACATCTTTACGAC346050.19886448694025508No Hit
GGGTGAAACCGACTAT334590.19227877094448764No Hit
AGGGACCCTAATCCTG330720.1900547987888489No Hit
TCTTGAACTCGTACAT327740.1883422827620263No Hit
TAACGAGCTTACTTTC322130.18511838514106163No Hit
TACGTGTGAAACGCAG321090.18452072854109666No Hit
CGGATGAGAACAGGCA319700.18372193750845126No Hit
TACCACCTGAAACATG318200.18285993279696336No Hit
AGCTTGTGAACCGTGC311210.1788429908414298No Hit
ACTTATCTGTCAGGTT303150.17421115219170155No Hit
CATGATGTGCCTAGTA303000.17412495172055278No Hit
AACTAGCACTGCCACG293920.1689069498670128No Hit
ACGTGTTGATCTTTGT278370.15997083435792173No Hit
TCGGACCGAATGTCCA277030.1592007768156592No Hit
AACTACGACATTGCCA271260.15588493202546913No Hit
TGGTAGGGATGTGCTA270280.15532175561396372No Hit
GTAGATAGAGCTGAGA268100.154068975433268No Hit
AACGAACCTGACACCC265690.1526840211968108No Hit
CGTCGTACTTGCGCCA263430.15138526743150238No Hit
GGTAGTTTGTCCAGCG260810.14987963253543685No Hit
CTCGGATCTAGTACGG259310.14901762782394898No Hit
TTTAGGGACTGGGCAA255710.14694881651637806No Hit
CCTTCAGTGCAACGCC253600.14573626322221844No Hit
AGTTTGCGAGCAGGAT250540.14397777361078315No Hit
TGAGGAGTGGTCCCAG247320.1421273368301225No Hit
TACCACCTGTACTTTC247290.14211009673589273No Hit
CTTTAACTGTTCGCCT240240.1380586745918997No Hit
AGAACGCGACACAGTT229440.13185224066918688No Hit
GCCATAATGACCGTTA228310.1312028637865327No Hit
CAGGCCATGTCGTAGT227080.13049601992311263No Hit
CTTACATTGGAAGGCT225520.12959953502316524No Hit
GAGAAGAGATGCTACA225390.1295248279481696No Hit
GGGATGTGATGCATGT222470.12784679210980654No Hit
GAAAGTCCTTATCTGG222380.12779507182711725No Hit
CGATCGGTGCGAGGAT218280.12543892561571704No Hit
TGCAATGACTCCGTAA216150.12421487892540423No Hit
CAAAGACCTGCTTTGA215740.12397926430426422No Hit
GAAGGACACCCAGCTG214350.12318047327161878No Hit
GGACCACTGGCTAGAG208890.12004277612180288No Hit
GCTGGACACATTGGTC207890.11946810631414427No Hit
AATTCTGCTTAGCGAG206600.1187267822622647No Hit
CCGTTACTGTTGGTTG205950.11835324688728663No Hit
AACTACGACGCTGTTA204320.11741653510080313No Hit
TCCTCATCTTTCTGGA202960.11663498416238745No Hit
GGCTTAAACTGCATCA198320.11396851625485158No Hit
TGTCTTCCTGAAATCA194820.11195717192804651No Hit
TCGTAGAACCGCACTG192960.11088828608580154No Hit
GCGTTTAGAAGTCTAC192180.11044004363582782No Hit
CCCACCTCTTGACCTA189930.109147036568596No Hit
TCCGTAAGATTACGCA189530.10891716864553257No Hit
ATGGCCGTGGGCCAAA188580.1083712323282569No Hit
ATAGCCAACATCTGTG187210.10758393469176464No Hit
CCTGAGGACTGACTCA185320.1064978087552899No Hit
ACAAGACCTAAGCTAA184500.10602657951300987No Hit
TATGGGTACCCTTTGC183630.10552661678034689No Hit
AGGTGATCTGTTAGTC179910.10338884509585693No Hit
GCGTTAATGGTACGTT175250.10071088379216792No Hit
GGCTTCTACATCAGCT174390.10021666775758152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)