FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004947888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3792269
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG361908195.43312987554417No Hit
GGCCAGGG634201.6723497199170205ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA432341.1400562565577494No Hit
GGACAGGC78790.2077647972757207No Hit
GGACACGG64720.17066299885372055No Hit
GGAAAGGG53720.1416566177135641No Hit
CGACAGGG52130.13746387716694147No Hit
GGACAGCG48110.12686336333208428No Hit
GCACAGGG42680.11254475882380706No Hit
GGTCAGGG39710.10471303591596483No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)