FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004947893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17622436
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG353300.2004830660187956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG31400.09.8980745
TAGACAG48000.09.7124855
TATACCG2253.438212E-68.7110985
GTATTAG23250.08.4293771
GTGTAGG24500.08.3992711
GTGTATA53650.08.3757881
ACTGATC30800.08.3649788
CTACACT29200.08.3419554
TACACTG38200.08.3193595
TAGACCG3201.1632437E-88.3124885
CTATACG3201.1643351E-88.3119924
TCTATAC22700.08.1399393
TAGACTG27650.08.1012545
CTAGACA45350.08.08770754
ATACTGT40450.07.9246516
GTGTAAG29150.07.9238411
TCTACAC25200.07.887893
CTAAGAC23750.07.8390083
TCTAGAC24700.07.76419973
TACACAG47300.07.7547455
Produced by FastQC (version 0.11.5)