FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004947908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004947908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3823140
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT394001.0305664976956115No Hit
TGCTGCTACCGTTCTC390221.020679336880156No Hit
AAACGACCTCTTCAAG370670.9695433596467825No Hit
ATGGGTTACAATCGAA362800.9489581862029641No Hit
TATGCTTTGGACTGTT356860.9334212192072484No Hit
AAAGCGGACTCGGGCT336800.8809512599590912No Hit
GCAGGTCTGGCTGTTA330880.8654666059835633No Hit
TCCAAAGTGCTCACGG315600.825499458560241No Hit
TAGTGCATGAACCCGC301740.789246535570238No Hit
GAAGTATGAACAGGTG295310.7724278995799264No Hit
TAACTCACTGGTGTCC284490.7441265556584378No Hit
TGTGGCTCTTACGATC283310.7410400874673697No Hit
TTCCCATCTCTTTGTT251730.658437828591158No Hit
TCACCTGACTCAATGC243700.6374341509858389No Hit
GGAACTACTACCAAGG236860.6195430980816816No Hit
TGAGAATGAGCAGTTT233510.6107806672002595No Hit
ATGTCCAGAGTCGGTC231850.6064386865246891No Hit
GCCTCTTGATCTGTGA230110.6018874537683685No Hit
CTAGAGACTAATCTGC230010.6016258886674304No Hit
GAAGTAATGAGTCTGT225730.5904309023472852No Hit
TCGTATACTGCTAATC218150.5706042676961869No Hit
TTCGTCCGACCTGTGG217200.5681193992372762No Hit
TCACACAGATTGGGAG213720.5590169337246347No Hit
TGCGGTGACCTTAGTG213670.5588861511741657No Hit
ATTCCTAACTGCTACA211180.5523731801608103No Hit
CCATGATGATTCGGGC208470.5452847659253912No Hit
ACTGATTCTCGGCTCA208400.5451016703547346No Hit
TCCGCGAGAGAACAGC208140.5444216010922959No Hit
AGGTCGAGAAGGGAGG206840.5410212547801022No Hit
TGGACCTCTAGGGAGG194910.509816538238202No Hit
GCAATCATGGAACGTG192450.5033820367551279No Hit
TGGCCTGGACCCGAGT189550.4957966488279268No Hit
ACGAGGATGGAGCTGA174240.4557510318743232No Hit
TTCGATGGAAATCCTG172530.45127826864828385No Hit
AAGGTGCGAACAGATA165500.4328902420523444No Hit
TTTCGTAACAGTACGG163610.42794666164461675No Hit
TCCCAGACTGTCTTGA158820.4154176933096878No Hit
GCGGTTCGAGTACGTT157410.4117296253864624No Hit
TGGTCCTTGGAGAGGG157290.4114157472653369No Hit
TCTCTCTACTCTGGAT155260.40610597571629603No Hit
TCCTTCACTTGTCACA140410.3672635582270071No Hit
TCGCCGAGAAGGTAAT138960.3634708642634065No Hit
TTCGATGGACAGGTGA136530.3571148323106138No Hit
AGCTGCTCTTGTCACA135790.3551792505636728No Hit
TGATATCCTCGATGTT134890.35282516465523106No Hit
CTTTCGGTGCTTTGTT131800.3447428030362477No Hit
AACTCCCTGCTTCACC131040.3427549082691191No Hit
AGCCCAGTGAAAGTAC125090.32719178476330973No Hit
CCCTTGCCTCCTACGG122030.3191878926746078No Hit
TCGGCTTACCTCCTAC120810.31599679844316453No Hit
ATGGCCGTGATACGCT119920.31366886904481656No Hit
AACCCTCACGCGGTTC118660.3103731487729981No Hit
TGTTTGTTGCAAGTTC114460.2993874145336033No Hit
AAACACGCTGCAGTTT114100.29844578017022655No Hit
GGGGGGGGGGGGGGGG113840.29776571090778786No Hit
CTAACTGGAACCTTGA112890.2952808424488771No Hit
TACAACGTGAGCAGGT109380.28609990740595426No Hit
AAATAGGACTCTATCT107780.2819148657909467No Hit
TGGCCTTTGCGAAAGC106990.27984850149353674No Hit
CAATGTGTGGTTGACT105930.2770759114235942No Hit
ACAGCCGACAAACATG105850.2768666593428438No Hit
CTTTCGGTGAGCCATT102590.26833963705226593No Hit
TCAAAGGCTCTTAGTG101920.2665871508759815No Hit
ACCCTTGTGGGCCATC101570.2656716730226986No Hit
GGACATTTGGCCGCTA97350.25463362576311616No Hit
TGAACTGTGTGCTGGC97060.25387508697039607No Hit
CAGCCGATGTAATAGG95350.24940232374435672No Hit
AGTGTTCGATTCGCCT92720.24252316158968806No Hit
GAGGTTGGAGGAATAT90880.23771036373242938No Hit
GATAAGAACATTGGTC86570.22643690788200274No Hit
GTGCTAATGGGAGTCA83110.21738675538954888No Hit
GGCTTGCGAATACGCT81510.21320171377454136No Hit
GTAAAGCCTGAAATCA81020.21192004477994528No Hit
CGACGTTCTTGCTACA80860.2115015406184445No Hit
TTGGGAGGAATCTGTG79110.20692415135203No Hit
TGTACTGGAAATGCTC78820.20616561255930987No Hit
GGAACATCTTTACGAC75460.19737702516779404No Hit
TCTTGAACTCGTACAT71990.18830071616524638No Hit
AGGGACCCTAATCCTG71330.18657438649905575No Hit
GGGTGAAACCGACTAT71190.18620819535774258No Hit
TAACGAGCTTACTTTC71050.18584200421642944No Hit
CGGATGAGAACAGGCA70420.1841941440805202No Hit
TACCACCTGAAACATG69050.18061070219766998No Hit
TACGTGTGAAACGCAG68380.17885821602138555No Hit
CATGATGTGCCTAGTA66710.17449007883572143No Hit
AGCTTGTGAACCGTGC66070.1728160621897184No Hit
ACTTATCTGTCAGGTT64680.1691803072866806No Hit
AACTAGCACTGCCACG64240.1680294208425535No Hit
ACGTGTTGATCTTTGT62250.16282427533388785No Hit
TCGGACCGAATGTCCA59740.15625899130034474No Hit
AACTACGACATTGCCA59580.155840487138844No Hit
GTAGATAGAGCTGAGA58950.15419262700293476No Hit
CGTCGTACTTGCGCCA58200.15223088874589996No Hit
TGGTAGGGATGTGCTA58090.15194316713486822No Hit
CTCGGATCTAGTACGG57250.14974602028698922No Hit
AACGAACCTGACACCC56580.14799353411070482No Hit
TTTAGGGACTGGGCAA55400.14490706591963673No Hit
TACCACCTGTACTTTC54630.14289301464241436No Hit
AGTTTGCGAGCAGGAT54510.14257913652128879No Hit
GGTAGTTTGTCCAGCG54130.1415851891377245No Hit
CCTTCAGTGCAACGCC53810.14074818081472298No Hit
CTTTAACTGTTCGCCT53170.13907416416871995No Hit
TGAGGAGTGGTCCCAG53130.13896953812834476No Hit
CGATCGGTGCGAGGAT51000.13339820147836595No Hit
AGAACGCGACACAGTT50050.1309133330194552No Hit
GAGAAGAGATGCTACA49580.12968397704504675No Hit
GAAAGTCCTTATCTGG49050.1282976820100755No Hit
CAAAGACCTGCTTTGA48460.12675444791454146No Hit
CTTACATTGGAAGGCT48280.1262836307328531No Hit
CAGGCCATGTCGTAGT47390.12395570133450515No Hit
GCCATAATGACCGTTA47050.12306637999131603No Hit
GGGATGTGATGCATGT46860.12256940629953388No Hit
TGCAATGACTCCGTAA46250.12097385918381227No Hit
GAAGGACACCCAGCTG45710.1195614076387472No Hit
GCTGGACACATTGGTC45360.1186459297854643No Hit
TCCTCATCTTTCTGGA45180.11817511260377596No Hit
AACTACGACGCTGTTA45070.11788739099274417No Hit
AATTCTGCTTAGCGAG44470.11631800038711636No Hit
CCGTTACTGTTGGTTG44060.11524558347327067No Hit
TGTCTTCCTGAAATCA42870.11213295877210878No Hit
GGCTTAAACTGCATCA42730.11176676763079564No Hit
TCGTAGAACCGCACTG42410.11092975930779411No Hit
CCCACCTCTTGACCTA42020.10990965541413603No Hit
ATGGCCGTGGGCCAAA41100.10750325648550668No Hit
ATAGCCAACATCTGTG41070.10742478695522528No Hit
ACAAGACCTAAGCTAA40810.10674471769278657No Hit
CCTGAGGACTGACTCA40630.1062739005110982No Hit
GCGTTTAGAAGTCTAC40540.10603849192025404No Hit
TCCGTAAGATTACGCA40450.10580308332940987No Hit
AGGTGATCTGTTAGTC40270.1053322661477215No Hit
TATGGGTACCCTTTGC39500.10331821487049911No Hit
GGACCACTGGCTAGAG39030.10208885889609065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)